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New insights into the post-translational modification of multiple phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and monoubiquitination during sorghum seed development and germination

Opened Access New insights into the post-translational modification of multiple phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and monoubiquitination during sorghum seed development and germination

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Autor: Ruiz Ballesta, Isabel
Gandullo Tovar, Jacinto Manuel
Wang, Liqun
Plaxton, William Charles
Echevarría Ruiz de Vargas, Cristina
Departamento: Universidad de Sevilla. Departamento de Biología Vegetal y Ecología
Fecha: 2016
Publicado en: Journal of Experimental Botany, 67 (11), 3523-3536..
Tipo de documento: Artículo
Resumen: Phosphoenolpyruvate carboxylase (PEPC; E.C. 4.1.1.31) was characterized in developing and germinating sorghum seeds, focusing on the transcript and polypeptide abundance of multiple plant-type phosphoenolpyruvate carboxylase (PTPC) genes, and the post-translational modification of each isoenzyme by phosphorylation versus monoubiquitination during germination. We observed high levels of SbPPC4 (Sb07g014960) transcripts during early development (stage I), and extensive transcript abundance of SbPPC2 (Sb02g021090) and SbPPC3 (Sb04g008720) throughout the entire life cycle of the seed. Although tandem mass spectrometry (MS) analysis of immunopurified PTPC indicated that four different PTPC isoenzymes were expressed in the developing and germinating seeds, SbPPC3 was the most abundant isozyme of the developing seed, and of the embryo and the aleurone layer of germinating seeds. In vivo phosphorylation of the different PTPC isoenzymes at their conserved N-terminal seryl phosphorylation site ...
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Cita: Ruiz Ballesta, I., Gandullo Tovar, J.M., Wang, i., Plaxton, W.C. y Echevarría Ruiz de Vargas, C. (2016). New insights into the post-translational modification of multiple phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and monoubiquitination during sorghum seed development and germination. Journal of Experimental Botany, 67 (11), 3523-3536..
Tamaño: 3.883Mb
Formato: PDF

URI: http://hdl.handle.net/11441/61044

DOI: 10.1093/jxb/erw186

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