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Actin cytoskeletal organization in human osteoblasts grown on different dental titanium implant surfaces


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dc.creator Salido Peracaula, Mercedes es
dc.creator Vilches Pérez, José Ignacio es
dc.creator Gutiérrez Pérez, José Luis es
dc.creator Vilches Troya, José es 2017-05-16T09:10:40Z 2017-05-16T09:10:40Z 2007-10-01
dc.identifier.issn 02133911 es
dc.description.abstract The understanding of the cellular basis of osteoblastic cell-biomaterial interaction is crucial to the analysis of the mechanism of osseointegration. Cell adhesion is a complex process that is dependent on the cell types and on the surface microtopography and chemistry of the substrate. We have studied the role of microtopography in modulating cell adhesion, in vitro, using a human osteoblastic cell line for the assessment of actin cytoskeletal organization. Through application of CLSM combining reflection and fluorescence, 2D or 3D images of cytoskeleton were obtained. On smooth surfaces, Ti CP machined, predominantly planar bone cells with an axial ratio of 1.1 were randomly oriented, with stress fibers running in all directions, and thin filopodia. On T iCP Osseotite ® surfaces the osteoblastic cells conformed to the irregular terrain of the sustrate with focal adhesion sites only established on the relative topographical peaks separated for a longer distance than in the machined surface, and defined wide lamellopodia and long filopodia, with enhanced expression of stress fibers, forming large clear focal contacts with the rough surface. The cytoskeletal organization of cells cultured on rough titanium supports an active role for the biomaterial surface in the events that govern osteoblastic cell adhesion. The results enforce the role of the rough sustrate surface in affecting osteoblastic cell adhesion and provide valuable information for the design of material surfaces that are required for the development of an appropriate osteogenic surface for osteoblastic anchorage, compared to machined surface, in dental implants. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Universidad de Murcia. Departamento de Biología Celular e Histología es
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.subject Osteoblasts es
dc.subject Cytoskeleton es
dc.subject Dental implants es
dc.subject T itanium es
dc.subject Microtopography es
dc.title Actin cytoskeletal organization in human osteoblasts grown on different dental titanium implant surfaces es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Estomatología es
idus.format.extent 10 es
dc.journaltitle Histology and Histopathology es
dc.publication.volumen 22 es
dc.publication.issue 10-12 es
dc.publication.initialPage 1355 es
dc.publication.endPage 1364 es
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