dc.creator | Cebolla, Ángel | es |
dc.creator | Sousa Martín, Carolina | es |
dc.creator | Lorenzo, Víctor de | es |
dc.date.accessioned | 2016-06-07T10:53:35Z | |
dc.date.available | 2016-06-07T10:53:35Z | |
dc.date.issued | 1997 | |
dc.identifier.citation | Cebolla, Á., Sousa Martín, C. y Lorenzo, V.d. (1997). Effector specificity mutants of the transcriptional activator NahR of naphthalene degrading Pseudomonas define protein sites involved in binding of aromatic inducers. Journal of Biological Chemistry, 272 (7), 3986-3992. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.uri | http://hdl.handle.net/11441/41988 | |
dc.description.abstract | This work reports a genetic analysis of the interactions
between NahR, the LysR-type regulator of the NAH
operons for biodegradation of naphthalene in Pseudomonas,
and its aromatic effectors. Six mutants encoding
NahR variants responsive to salicylate analogs such as
benzoate, which is not an inducer for the wild type regulator,
were isolated with a polymerase chain reactionbased
saturation mutagenesis protocol. Most mutants
displaying a specific change of effector profile bore single
amino acid substitutions within a short protein segment
of 60 residues located at the central portion of the
NahR sequence. Some of the protein variants exhibited
an increased affinity for salicylate and also for otherwise
suboptimal effectors, with apparent Ks
* values
5–100-fold lower than those of the wild type NahR protein.
In addition, all mutants were activated by inducers
bearing novel substituents at positions 1 or 2 of the
aromatic ring and displayed also an enhanced tolerance
to changes at positions 3 and 4. Correlation between
mutations in NahR and the structures of the new effectors
suggested that protein sites Met116, Arg132, Asn169,
and Arg248 are involved in effector recognition and binding
during the earlier steps of the process leading to
transcriptional activation of cognate NAH promoters | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Biochemistry and Molecular Biology Inc. | es |
dc.relation.ispartof | Journal of Biological Chemistry, 272 (7), 3986-3992. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Effector specificity mutants of the transcriptional activator NahR of naphthalene degrading Pseudomonas define protein sites involved in binding of aromatic inducers | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | http://0-www.jbc.org.fama.us.es/content/272/7/3986 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.272.7.3986 | |
dc.identifier.doi | 10.1074/jbc.272.7.3986 | |
idus.format.extent | 8 p. | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 272 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 3986 | es |
dc.publication.endPage | 3992 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/41988 | |