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Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity

 

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dc.creator Cebolla, Ángel es
dc.creator Sousa Martín, Carolina es
dc.creator Lorenzo, Víctor de es
dc.date.accessioned 2016-06-07T09:29:45Z
dc.date.available 2016-06-07T09:29:45Z
dc.date.issued 2001
dc.identifier.citation Cebolla, Á., Sousa Martín, C. y Lorenzo, V.d. (2001). Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity. Nucleic Acids Research, 29 (3), 759-766.
dc.identifier.issn 0305-1048 es
dc.identifier.uri http://hdl.handle.net/11441/41958
dc.description.abstract Cascade regulatory circuits have been described that control numerous cell processes, and may provide models for the design of artificial circuits with novel properties. Here we describe the design of a transcriptional regulatory cascade to amplify the cell response to a given signal. We used the salicylate-responsive activators of Pseudomonas putida NahR of the naphthalene degradation plasmid NAH7 and XyIS2, a mutant regulator of the TOL plasmid for catabolism of m-xylene and their respective cognate promoters Psal and Pm. Control of the expression of xylS2 with the nahR/Psal system permitted either their selective activation with specific effectors for each protein or the simultaneous activation of both of them with salicylate. When cells face the common effector of the two regulators, both the increase in XylS2 concentration and the stimulation of its activity act synergistically on the Pm promoter, amplifying the gene expression capacity by at least one order of magnitude with respect to the individual systems. By changing the hierarchy of regulators, we showed that the specific features of the downstream regulator were crucial for the amplification effect. Directed changes in the effector profile of the regulators allowed the extension of the amplifying system to other molecular signals es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher Oxford University Press es
dc.relation.ispartof Nucleic Acids Research, 29 (3), 759-766.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Rational design of a bacterial transcriptional cascade for amplifying gene expression capacity es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología y Parasitología es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Genética es
dc.relation.publisherversion 10.1093/nar/29.3.759 es
dc.identifier.doi http://dx.doi.org/10.1093/nar/29.3.759 es
idus.format.extent 8 p. es
dc.journaltitle Nucleic Acids Research es
dc.publication.volumen 29 es
dc.publication.issue 3 es
dc.publication.initialPage 759 es
dc.publication.endPage 766 es
dc.identifier.idus https://idus.us.es/xmlui/handle/11441/41958
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