Autor: |
Zamora, Leydis
Fernández Garayzábal, José F. Sánchez-Porro Álvarez, Cristina Palacios, Maria Ángel Moore, Edward R. B. Domínguez, Lucas Ventosa Ucero, Antonio Vela, Ana I. |
Departamento: | Universidad de Sevilla. Departamento de Microbiología y Parasitología |
Fecha: | 2013 |
Publicado en: | PLoS One, 8 (6), 1-7. |
Tipo de documento: | Artículo |
Resumen: |
Five strains (1126-1H-08T, 51B-09, 986-08, 1084B-08 and 424-08) were isolated from diseased rainbow trout. Cells were Gram-negative rods, 0.7 mm wide and 3 mm long, non-endospore-forming, catalase and oxidase positive. Colonies were circular, yellow-pigmented, smooth and entire on TGE agar after 72 hours incubation at 25uC. They grew in a temperature range between 15uC to 30uC, but they did not grow at 37uCor 42uC. Based on 16S rRNA gene sequence analysis, the isolates belonged to the genus Flavobacterium. Strain 1126-1H-08T exhibited the highest levels of similarity with Flavobacterium oncorhynchi CECT 7678T and Flavobacterium pectinovorum DSM 6368T (98.5% and 97.9% sequence similarity, respectively). DNA–DNA hybridization values were 87 to 99% among the five isolates and ranged from 21 to 48% between strain 1126-1H- 08T, selected as a representative isolate, and the type strains of Flavobacterium oncorhynchi CECT 7678T and other phylogenetic related Flavobacterium species. The DNA G... [Ver más] |
URI: http://hdl.handle.net/11441/17832
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