Artículo
Identification of the direct regulon of NtcA during early acclimation to nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803
Autor/es | Giner Lamia, Joaquín
Robles Rengel, Rocio Hernández Prieto, Miguel Ángel Muro Pastor, María Isabel Florencio Bellido, Francisco Javier Futschik, Matthias E. |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2017 |
Fecha de depósito | 2020-05-13 |
Publicado en |
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Resumen | In cyanobacteria, nitrogen homeostasis is maintained by an intricate regulatory network around transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, its regulon remains ... In cyanobacteria, nitrogen homeostasis is maintained by an intricate regulatory network around transcription factor NtcA. Although mechanisms controlling NtcA activity appear to be well understood, its regulon remains poorly defined. To determine the NtcA regulon during the early stages of nitrogen starvation for the model cyanobacterium Synechocystis sp. PCC 6803, we performed chromatin immunoprecipitation, followed by sequencing (ChIP-seq), in parallel with transcriptome analysis (RNA-seq). Through combining these methods, we determined 51 genes activated and 28 repressed directly by NtcA. In addition to genes associated with nitrogen and carbon metabolism, a considerable number of genes without current functional annotation were among direct targets providing a rich reservoir for further studies. The NtcA regulon also included eight non-coding RNAs, of which Ncr1071, Syr6 and NsiR7 were experimentally validated, and their putative targets were computationally predicted. Surprisingly, we found substantial NtcA binding associated with delayed expression changes indicating that NtcA can reside in a poised state controlled by other factors. Indeed, a role of PipX as modulating factor in nitrogen regulation was confirmed for selected NtcA-targets. We suggest that the indicated poised state of NtcA enables a more differentiated response to nitrogen limitation and can be advantageous in native habitats of Synechocystis. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Junta de Andalucía European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) |
Identificador del proyecto | BFU2013– 41712
BIO2016–75634 BIO-284 P12-BIO-1119 |
Cita | Giner Lamia, J., Robles Rengel, R., Hernández Prieto, M.Á., Muro Pastor, M.I., Florencio Bellido, F.J. y Futschik, M.E. (2017). Identification of the direct regulon of NtcA during early acclimation to nitrogen starvation in the cyanobacterium Synechocystis sp. PCC 6803. Nucleic Acids Research, 45 (20), 11800-11820. |
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