dc.creator | Wiedorn, Max O. | es |
dc.creator | Awel, Salah | es |
dc.creator | Morgan, Andrew J. | es |
dc.creator | Ayyer, Kartik | es |
dc.creator | Gevorkov, Yaroslav | es |
dc.creator | Cruz-Mazo, Francisco | es |
dc.creator | Gañán-Calvo, Alfonso M. | es |
dc.date.accessioned | 2020-04-13T20:08:41Z | |
dc.date.available | 2020-04-13T20:08:41Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Wiedorn, M.O., Awel, S., Morgan, A.J., Ayyer, K., Gevorkov, Y., Cruz-Mazo, F. y Gañán-Calvo, A.M. (2018). Rapid sample delivery for megahertz serial crystallography at X-ray FELs. IUCrJ, 5, 574-584. | |
dc.identifier.issn | ESSN: 2052-2525 | es |
dc.identifier.uri | https://hdl.handle.net/11441/95087 | |
dc.description.abstract | Liquid microjets are a common means of delivering protein crystals to the focus
of X-ray free-electron lasers (FELs) for serial femtosecond crystallography
measurements. The high X-ray intensity in the focus initiates an explosion of the
microjet and sample. With the advent of X-ray FELs with megahertz rates, the
typical velocities of these jets must be increased significantly in order to
replenish the damaged material in time for the subsequent measurement with
the next X-ray pulse. This work reports the results of a megahertz serial
diffraction experiment at the FLASH FEL facility using 4.3 nm radiation. The
operation of gas-dynamic nozzles that produce liquid microjets with velocities
greater than 80 m s1 was demonstrated. Furthermore, this article provides
optical images of X-ray-induced explosions together with Bragg diffraction from
protein microcrystals exposed to trains of X-ray pulses repeating at rates of up
to 4.5 MHz. The results indicate the feasibility for megahertz serial crystallography measurements with hard X-rays and give guidance for the design of
such experiments. | es |
dc.description.sponsorship | Unión Europea 7PM / 2007-2013 | es |
dc.description.sponsorship | Consejo de Investigación de Australia DP170100131 | es |
dc.description.sponsorship | Ministerio de Economía, Industria y Competitividad DPI2016-78887-C3-1-R | es |
dc.description.sponsorship | National Science Foundation "BioXFEL" (1231306) | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.publisher | International Union of Crystallography | es |
dc.relation.ispartof | IUCrJ, 5, 574-584. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | X-ray free-electron lasers | es |
dc.subject | FELs | es |
dc.subject | X-ray FEL pulse trains | es |
dc.subject | Megahertz repetition rates | es |
dc.title | Rapid sample delivery for megahertz serial crystallography at X-ray FELs | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Ingeniería Aeroespacial y Mecánica de Fluidos | es |
dc.relation.projectID | 7PM / 2007-2013 | es |
dc.relation.projectID | DP170100131 | es |
dc.relation.projectID | DPI2016-78887-C3-1-R | es |
dc.relation.projectID | "BioXFEL" (1231306) | es |
dc.relation.publisherversion | https://doi.org/10.1107/S2052252518008369 | es |
dc.identifier.doi | 10.1107/S2052252518008369 | es |
dc.journaltitle | IUCrJ | es |
dc.publication.volumen | 5 | es |
dc.publication.initialPage | 574 | es |
dc.publication.endPage | 584 | es |
dc.identifier.sisius | 21724750 | |
dc.contributor.funder | European Union (UE) | es |
dc.contributor.funder | Australian Research Council | es |
dc.contributor.funder | Ministerio de Economia, Industria y Competitividad (MINECO). España | es |
dc.contributor.funder | National Science Foundation (NSF). United States | es |