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dc.creatorVaquero Sedas, María Isabeles
dc.creatorVega Palas, Miguel Ángeles
dc.date.accessioned2020-03-31T08:26:05Z
dc.date.available2020-03-31T08:26:05Z
dc.date.issued2019
dc.identifier.citationVaquero Sedas, M.I. y Vega Palas, M.Á. (2019). Assessing the Epigenetic Status of Human Telomeres. Cells, 8 (9), 1-10.
dc.identifier.issn2073-4409 (impreso, electrónico)es
dc.identifier.urihttps://hdl.handle.net/11441/94714
dc.description.abstractThe epigenetic modifications of human telomeres play a relevant role in telomere functions and cell proliferation. Therefore, their study is becoming an issue of major interest. These epigenetic modifications are usually analyzed by microscopy or by chromatin immunoprecipitation (ChIP). However, these analyses could be challenged by subtelomeres and/or interstitial telomeric sequences (ITSs). Whereas telomeres and subtelomeres cannot be differentiated by microscopy techniques, telomeres and ITSs might not be differentiated in ChIP analyses. In addition, ChIP analyses of telomeres should be properly controlled. Hence, studies focusing on the epigenetic features of human telomeres have to be carefully designed and interpreted. Here, we present a comprehensive discussion on how subtelomeres and ITSs might influence studies of human telomere epigenetics. We specially focus on the influence of ITSs and some experimental aspects of the ChIP technique on ChIP analyses. In addition, we propose a specific pipeline to accurately perform these studies. This pipeline is very simple and can be applied to a wide variety of cells, including cancer cells. Since the epigenetic status of telomeres could influence cancer cells proliferation, this pipeline might help design precise epigenetic treatments for specific cancer types.es
dc.description.sponsorshipSpanish Agency of Researches
dc.description.sponsorshipEuropean Fund for Regional Development European Union BIO2016-78955-Pes
dc.formatapplication/pdfes
dc.format.extent10 p.es
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofCells, 8 (9), 1-10.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTelomereses
dc.subjectEpigeneticses
dc.subjectChromatin immunoprecipitationes
dc.subjectMicroscopyes
dc.subjectHumanes
dc.subjectCanceres
dc.titleAssessing the Epigenetic Status of Human Telomereses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.projectIDBIO2016-78955-Pes
dc.relation.publisherversionhttp://dx.doi.org/10.3390/cells8091050es
dc.identifier.doi10.3390/cells8091050es
dc.journaltitleCellses
dc.publication.volumen8es
dc.publication.issue9es
dc.publication.initialPage1es
dc.publication.endPage10es
dc.contributor.funderAgencia Estatal de Investigación. Españaes
dc.contributor.funderEuropean Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)es

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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