dc.creator | Martínez Negro, María | es |
dc.creator | Sánchez Arribas, Natalia | es |
dc.creator | Guerrero Martínez, Andrés | es |
dc.creator | Moyá Morán, María Luisa | es |
dc.creator | Tros de Llarduya, Conchita | es |
dc.creator | Mendicuti, Francisco | es |
dc.creator | Aicart, Emilio | es |
dc.creator | Junquera, Elena | es |
dc.date.accessioned | 2019-12-02T10:00:41Z | |
dc.date.available | 2019-12-02T10:00:41Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Martínez Negro, M., Sánchez Arribas, N., Guerrero Martínez, A., Moyá Morán, M.L., Tros de Llarduya, C., Mendicuti, F.,...,Junquera, E. (2019). A Non-Viral Plasmid DNA Delivery System Consisting on a Lysine-Derived Cationic Lipid Mixed with a Fusogenic Lipid. Pharmaceutics, 11 (12), 1-16. | |
dc.identifier.issn | 1999-4923 | es |
dc.identifier.uri | https://hdl.handle.net/11441/90656 | |
dc.description.abstract | The insertion of biocompatible amino acid moieties in non-viral gene nanocarriers is an attractive approach that has been recently gaining interest. In this work, a cationic lipid, consisting of a lysine-derived moiety linked to a C12 chain (LYCl) was combined with a common fusogenic helper lipid (DOPE) and evaluated as a potential vehicle to transfect two plasmid DNAs (encoding green fluorescent protein GFP and luciferase) into COS-7 cells. A multidisciplinary approach has been followed: (i) biophysical characterization based on zeta potential, gel electrophoresis, small-angle X-ray scattering (SAXS), and cryo-transmission electronic microscopy (cryo-TEM); (ii) biological studies by fluorescence assisted cell sorting (FACS), luminometry, and cytotoxicity experiments; and (iii) a computational study of the formation of lipid bilayers and their subsequent stabilization with DNA. The results indicate that LYCl/DOPE nanocarriers are capable of compacting the pDNAs and protecting them efficiently against DNase I degradation, by forming Lα lyotropic liquid crystal phases, with an average size of ~200 nm and low polydispersity that facilitate the cellular uptake process. The computational results confirmed that the LYCl/DOPE lipid bilayers are stable and also capable of stabilizing DNA fragments via lipoplex formation, with dimensions consistent with experimental values. The optimum formulations (found at 20% of LYCl content) were able to complete the transfection process efficiently and with high cell viabilities, even improving the outcomes of the positive control Lipo2000* | es |
dc.description.sponsorship | España, MINECO (contract numbers CTQ2015-65972-R, CTQ2015-64425-C2-1-R, CTQ2015-64425-C2-2-R, CTQ2016-80600-P and RTI2018-095844-B-I00) | es |
dc.description.sponsorship | España, University Complutense of Madrid (project number UCMA05-33-010) | es |
dc.description.sponsorship | España, University of Alcalá (project number CCGP2017-EXP/027) | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Pharmaceutics, 11 (12), 1-16. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | compaction | es |
dc.subject | gene delivery | es |
dc.subject | lipoplex | es |
dc.subject | lysine-derived cationic lipid | es |
dc.subject | molecular dynamics | es |
dc.subject | multilamellar aggregates | es |
dc.subject | plasmid DNA | es |
dc.subject | protection | es |
dc.subject | transfection | es |
dc.title | A Non-Viral Plasmid DNA Delivery System Consisting on a Lysine-Derived Cationic Lipid Mixed with a Fusogenic Lipid | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Física | es |
dc.relation.projectID | contract numbers CTQ2015-65972-R, CTQ2015-64425-C2-1-R, CTQ2015-64425-C2-2-R, CTQ2016-80600-P and RTI2018-095844-B-I00) | es |
dc.relation.projectID | project number UCMA05-33-010 | es |
dc.relation.projectID | project number CCGP2017-EXP/027 | es |
dc.relation.publisherversion | http://dx.doi.org/10.3390/pharmaceutics11120632 | es |
dc.identifier.doi | 10.3390/pharmaceutics11120632 | es |
idus.format.extent | 16 p. | es |
dc.journaltitle | Pharmaceutics | es |
dc.publication.volumen | 11 | es |
dc.publication.issue | 12 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 16 | es |