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dc.creatorOlloqui Sariego, José Luises
dc.creatorMárquez Escudero, Inmaculadaes
dc.creatorFrutos Beltrán, Estrellaes
dc.creatorDíaz Moreno, Irenees
dc.creatorRosa Acosta, Miguel Ángel de laes
dc.creatorCalvente Pacheco, Juan Josées
dc.creatorAndreu Fondacabe, Rafael Jesúses
dc.creatorDíaz Quintana, Antonio Jesúses
dc.date.accessioned2019-11-13T16:09:04Z
dc.date.available2019-11-13T16:09:04Z
dc.date.issued2018
dc.identifier.citationOlloqui Sariego, J.L., Márquez Escudero, I., Frutos Beltrán, E., Díaz Moreno, I., Rosa Acosta, M.Á.d.l., Calvente Pacheco, J.J.,...,Díaz Quintana, A.J. (2018). Key Role of the Local Hydrophobicity in the East Patch of Plastocyanins on Their Thermal Stability and Redox Properties. ACS Omega, 3 (9), 11447-11454.
dc.identifier.issn2470-1343es
dc.identifier.urihttps://hdl.handle.net/11441/90190
dc.description.abstractUnderstanding the molecular basis of the thermal stability and functionality of redox proteins has important practical applications. Here, we show a distinct thermal dependence of the spectroscopic and electrochemical properties of two plastocyanins from the thermophilic cyanobacterium Phormidium laminosum and their mesophilic counterpart from Synechocystis sp. PCC 6803, despite the similarity of their molecular structures. To explore the origin of these differences, we have mimicked the local hydrophobicity in the east patch of the thermophilic protein by replacing a valine of the mesophilic plastocyanin by isoleucine. Interestingly, the resulting mutant approaches the thermal stability, redox thermodynamics, and dynamic coupling of the flexible site motions of the thermophilic protein, indicating the existence of a close connection between the hydrophobic packing of the east patch region of plastocyanin and the functional control and stability of the oxidized and reduced forms of the proteines
dc.description.sponsorshipSpanish Ministry of Economy, Industry and Competitiveness (BFU2015-71017/BMC)es
dc.description.sponsorshipSpanish, Ramon Areces Foundation (2015−2017)es
dc.description.sponsorshipAndalusian Government (BIO-198)es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Chemical Societyes
dc.relation.ispartofACS Omega, 3 (9), 11447-11454.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleKey Role of the Local Hydrophobicity in the East Patch of Plastocyanins on Their Thermal Stability and Redox Propertieses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Química Físicaes
dc.relation.projectIDBFU2015-71017/BMCes
dc.relation.projectID2015−2017es
dc.relation.projectIDBIO-198es
dc.relation.publisherversionhttp://dx.doi.org/10.1021/acsomega.8b01612es
dc.identifier.doi10.1021/acsomega.8b01612es
idus.format.extent8 p.es
dc.journaltitleACS Omegaes
dc.publication.volumen3es
dc.publication.issue9es
dc.publication.initialPage11447es
dc.publication.endPage11454es

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