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Artículo
Rat1p maintains RNA polymerase II CTD phosphorylation balance
dc.creator | Jimeno González, Silvia | es |
dc.creator | Schmid, Manfred | es |
dc.creator | Malagón Cobos, Francisco | es |
dc.creator | Haaning, Line Lindegaard | es |
dc.creator | Jensen, Torben Heick | es |
dc.date.accessioned | 2019-02-06T12:28:19Z | |
dc.date.available | 2019-02-06T12:28:19Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Jimeno González, S., Schmid, M., Malagón Cobos, F., Haaning, L.L. y Jensen, T.H. (2014). Rat1p maintains RNA polymerase II CTD phosphorylation balance. RNA, 20, 551-558. | |
dc.identifier.issn | 1355-8382 (impreso) | es |
dc.identifier.issn | 1469-9001 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/82624 | |
dc.description.abstract | In S. cerevisiae, the 5′-3′ exonuclease Rat1p partakes in transcription termination. Although Rat1p-mediated RNA degradation has been suggested to play a role for this activity, the exact mechanisms by which Rat1p helps release RNA polymerase II (RNAPII) from the DNA template are poorly understood. Here we describe a function of Rat1p in regulating phosphorylation levels of the C-terminal domain (CTD) of the largest RNAPII subunit, Rpb1p, during transcription elongation. The rat1-1 mutant exhibits highly elevated levels of CTD phosphorylation as well as RNAPII distribution and transcription termination defects. These phenotypes are all rescued by overexpression of the CTD phosphatase Fcp1p, suggesting a functional relationship between the absence of Rat1p activity, elevated CTD phosphorylation, and transcription defects. We also demonstrate that rat1-1 cells display increased RNAPII transcription kinetics, a feature that may contribute to the cellular phenotypes of the mutant. Consistently, the rat1-1 allele is synthetic lethal with the rpb1-E1103G mutation, causing increased RNAPII speed, and is suppressed by the rpb2-10 mutation, causing slowed transcription. Thus, Rat1p plays more complex roles in controlling transcription than previously thought. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Cold Spring Harbor Laboratory Press | es |
dc.relation.ispartof | RNA, 20, 551-558. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | RNA polymerase II | es |
dc.subject | CTD phosphorylation | es |
dc.subject | Transcription elongation | es |
dc.subject | Transcription termination | es |
dc.title | Rat1p maintains RNA polymerase II CTD phosphorylation balance | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1261/rna.041129.113 | es |
dc.identifier.doi | 10.1261/rna.041129.113 | es |
idus.format.extent | 9 p. | es |
dc.journaltitle | RNA | es |
dc.publication.volumen | 20 | es |
dc.publication.initialPage | 551 | es |
dc.publication.endPage | 558 | es |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Rat1p maintains.pdf | 402.6Kb | ![]() | Ver/ | |