dc.creator | García Calderón, Margarita | es |
dc.creator | Pérez Delgado de Torres, Carmen María | es |
dc.creator | Credali, Alfredo | es |
dc.creator | Vega Piqueres, José María | es |
dc.creator | Betti, Marco | es |
dc.creator | Márquez Cabeza, Antonio José | es |
dc.date.accessioned | 2019-01-10T11:47:05Z | |
dc.date.available | 2019-01-10T11:47:05Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | García Calderón, M., Pérez Delgado de Torres, C.M., Credali, A., Vega Piqueres, J.M., Betti, M. y Márquez Cabeza, A.J. (2017). Genes for asparagine metabolism in Lotus japonicus : differential expression and interconnection with photorespiration. BMC Genomics, 18, 781. | |
dc.identifier.issn | 1471-2164 | es |
dc.identifier.uri | https://hdl.handle.net/11441/81401 | |
dc.description.abstract | Background:
Asparagine is a very important nitrogen transport and storage compound in plants due to its high
nitrogen/carbon ratio and stability. Asparagine intracellu
lar concentration depends on a balance between asparagine
biosynthesis and degradation. The main enzymes involved in
asparagine metabolism are as
paragine synthetase (ASN),
asparaginase (NSE) and serine-glyoxylate aminotransfera
se (SGAT). The study of the genes encoding for these enzymes
in the model legume
Lotus japonicus
is of particular interest since it has been proposed that asparagine is the principal
molecule used to transport reduced nitrogen within the plant in most temperate legumes.
Results:
A differential expression of genes encoding for seve
ral enzymes involved in asparagine metabolism was
detected in
L. japonicus
. ASN is encoded by three genes,
LjASN1
was the most highly expressed in mature leaves while
LjASN2
expression was negligible and
LjASN3
showed a low expression in this organ, suggesting that
LjASN1
is the main
gene responsible for asparagine synthesis in mature leaves. In young leaves,
LjASN3
was the only ASN gene expressed
although at low levels, while all the three genes encoding for NSE were highly expressed, especially
LjNSE1
.Innodules,
LjASN2
and
LjNSE2
were the most highly expressed genes, suggesting an important role for these genes in this organ.
Several lines of evidence support the connection between asparagine metabolic genes and photorespiration in
L.
japonicus
: a) a mutant plant deficient in
LjNSE1
showed a dramatic decrease in the expression of the two genes
encoding for SGAT; b) expression of the genes involved in asparagine metabolism is altered in a photorespiratory
mutant lacking plastidic glutamine synthetase; c) a clustering analysis indicated a similar pattern of expression among
several genes involved in photorespiratory and asparagine metabolism, indicating a clear link between
LjASN1
and
LjSGAT
genes and photorespiration.
Conclusions:
The results obtained in this paper indicate the exis
tence of a differential expression of asparagine
metabolic genes in
L. japonicus
and point out the crucial relevance of particular genes in different organs.
Moreover, the data presented establish clear links betw
een asparagine and photorespiratory metabolic genes in
this plant. | es |
dc.description.sponsorship | Junta de Andalucía (P10-CVI- 6368) | es |
dc.description.sponsorship | FEDER-Ministerio de Economía y Competitividad (AGL 2014 – 54413-R) | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | BioMed Central | es |
dc.relation.ispartof | BMC Genomics, 18, 781. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Asparaginase genes | es |
dc.subject | Asparagine synthetase genes | es |
dc.subject | Lotus japonicus | es |
dc.subject | Serine-glyoxylate aminotransferase genes | es |
dc.title | Genes for asparagine metabolism in Lotus japonicus : differential expression and interconnection with photorespiration | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | P10-CVI- 6368 | es |
dc.relation.projectID | AGL 2014 – 54413-R | es |
dc.relation.publisherversion | https://doi.org/10.1186/s12864-017-4200-x | es |
dc.identifier.doi | 10.1186/s12864-017-4200-x | es |
idus.format.extent | 14 | es |
dc.journaltitle | BMC Genomics | es |
dc.publication.volumen | 18 | es |
dc.publication.initialPage | 781 | es |
dc.description.awardwinning | Premio Mensual Publicación Científica Destacada de la US. Facultad de Química | |