Artículo
Decorating a single giant DNA with gold nanoparticles
Autor/es | Carnerero Panduro, José María
Masuoka, Shinsuke Baba, Hikari Yoshikawa, Yuko Prado Gotor, Rafael Yoshikawa, Kenichi |
Departamento | Universidad de Sevilla. Departamento de Química Física |
Fecha de publicación | 2018 |
Fecha de depósito | 2018-10-09 |
Publicado en |
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Resumen | We decorated a single giant DNA (1.66 × 105 base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy ... We decorated a single giant DNA (1.66 × 105 base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, i.e., Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by ca. 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA skeleton. UV-visible measurements revealed the absence of any detectable change in surface plasmon resonance, suggesting that the gold nanoparticles assemble without the formation of a densely packed aggregate. CD measurements showed that the secondary structure of decorated DNA is still essentially the B-form. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España Universidad de Sevilla |
Identificador del proyecto | CTQ2016-78703-P
2010/00000762 15H02121 25103012 |
Cita | Carnerero Panduro, J.M., Masuoka, S., Baba, H., Yoshikawa, Y., Prado Gotor, R. y Yoshikawa, K. (2018). Decorating a single giant DNA with gold nanoparticles. RSC Advances, 8, 26571-26579. |
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