Decorating a single giant DNA with gold nanoparticles
|Author/s||Carnerero Panduro, José María
Prado Gotor, Rafael
|Department||Universidad de Sevilla. Departamento de Química Física|
|Abstract||We decorated a single giant DNA (1.66 × 105 base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy ...
We decorated a single giant DNA (1.66 × 105 base pairs) with gold nanoparticles through the simple procedure of mild warming, without denaturation of the DNA molecule. Single-molecule observation with fluorescence microscopy revealed that individual decorated DNA molecules stay in the bulk solution by avoiding aggregation and precipitation, and exhibit translational and conformational fluctuation, i.e., Brownian motion. An analysis of the intra-chain fluctuation of single DNA molecules revealed that the apparent spring constant and damping coefficient of a DNA chain increased by ca. 13- and 5-fold, respectively, upon decoration with gold nanoparticles. Observation by transmission electron microscopy revealed that gold nanoparticles were stably attached to the DNA skeleton. UV-visible measurements revealed the absence of any detectable change in surface plasmon resonance, suggesting that the gold nanoparticles assemble without the formation of a densely packed aggregate. CD measurements showed that the secondary structure of decorated DNA is still essentially the B-form.
|Funding agencies||Ministerio de Economía y Competitividad (MINECO). España
Universidad de Sevilla
|Citation||Carnerero Panduro, J.M., Masuoka, S., Baba, H., Yoshikawa, Y., Prado Gotor, R. y Yoshikawa, K. (2018). Decorating a single giant DNA with gold nanoparticles. RSC Advances, 8, 26571-26579.|