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dc.creatorCouso Liáñez, Inmaculada Concepciónes
dc.creatorPérez Pérez, María Estheres
dc.creatorMartínez Force, Enriquees
dc.creatorKim, Hee-Sikes
dc.creatorCrespo González, José Luises
dc.date.accessioned2018-06-15T09:17:04Z
dc.date.available2018-06-15T09:17:04Z
dc.date.issued2018
dc.identifier.citationCouso Liáñez, I.C., Pérez Pérez, M.E., Martínez Force, E., Kim, H. y Crespo González, J.L. (2018). Autophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonas. Journal of Experimental Botany, 69 (6), 1355-1367.
dc.identifier.issn1477-9145es
dc.identifier.urihttps://hdl.handle.net/11441/76249
dc.description.abstractAutophagy is an intracellular catabolic process that allows cells to recycle unneeded or damaged material to maintain cellular homeostasis. This highly dynamic process is characterized by the formation of double-membrane vesicles called autophagosomes, which engulf and deliver the cargo to the vacuole. Flow of material through the autophagy pathway and its degradation in the vacuole is known as autophagic flux, and reflects the autophagic degradation activity. A number of assays have been developed to determine autophagic flux in yeasts, mammals, and plants, but it has not been examined yet in algae. Here we analyzed autophagic flux in the model green alga Chlamydomonas reinhardtii. By monitoring specific autophagy markers such as ATG8 lipidation and using immunofluorescence and electron microscopy techniques, we show that concanamycin A, a vacuolar ATPase inhibitor, blocks autophagic flux in Chlamydomonas. Our results revealed that vacuolar lytic function is needed for the synthesis of triacylglycerols and the formation of lipid bodies in nitrogen- or phosphate-starved cells. Moreover, we found that concanamycin A treatment prevented the degradation of ribosomal proteins RPS6 and RPL37 under nitrogen or phosphate deprivation. These results indicate that autophagy might play an important role in the regulation of lipid metabolism and the recycling of ribosomal proteins under nutrient limitation in Chlamydomonases
dc.description.sponsorshipEspaña, MINECO BFU2015-68216-Pes
dc.description.sponsorshipEspaña, Junta de Andalucía CVI-7336 (to JLC), BIO2015-74432-JIN (to MEPP)es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherCompany of Biologistses
dc.relation.ispartofJournal of Experimental Botany, 69 (6), 1355-1367.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAutophagyes
dc.subjectChlamydomonases
dc.subjectConcanamycin Aes
dc.subjectLipid bodyes
dc.subjectNitrogenes
dc.subjectPhosphatees
dc.subjectRibosomal proteines
dc.subjectTriacylglyceroles
dc.titleAutophagic flux is required for the synthesis of triacylglycerols and ribosomal protein turnover in Chlamydomonases
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.projectIDBFU2015-68216-Pes
dc.relation.projectIDCVI-7336es
dc.relation.projectIDBIO2015-74432-JINes
dc.relation.publisherversionhttp://dx.doi.org/10.1093/jxb/erx372es
dc.identifier.doi10.1093/jxb/erx372es
idus.format.extent13 p.es
dc.journaltitleJournal of Experimental Botanyes
dc.publication.volumen69es
dc.publication.issue6es
dc.publication.initialPage1355es
dc.publication.endPage1367es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). España
dc.contributor.funderJunta de Andalucía

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