dc.creator | Ávila Medina, Javier | es |
dc.creator | Calderón Sánchez, Eva María | es |
dc.creator | González-Rodríguez, Patricia | es |
dc.creator | Monje Quiroga, Francisco | es |
dc.creator | Rosado, Juan Antonio | es |
dc.creator | Castellano Orozco, Antonio Gonzalo | es |
dc.creator | Ordóñez Fernández, José Antonio | es |
dc.creator | Smani Hajami, Tarik | es |
dc.date.accessioned | 2018-05-25T11:11:11Z | |
dc.date.available | 2018-05-25T11:11:11Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Ávila Medina, J., Calderón Sánchez, E.M., González-Rodríguez, P., Monje Quiroga, F., Rosado, J.A., Castellano Orozco, A.G.,...,Smani Hajami, T. (2016). Orai1 and TRPC1 Proteins Co-localize with CaV1.2 Channels to Form a Signal Complex in Vascular Smooth Muscle Cells. The Journal of Biological Chemistry, 291 (40), 21148-21159. | |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | https://hdl.handle.net/11441/75157 | |
dc.description.abstract | Voltage-dependent CaV1.2 L-type Ca2 channels (LTCC) are
the main route for calcium entry in vascular smooth muscle cells
(VSMC). Several studies have also determined the relevant role
of store-operated Ca2 channels (SOCC) in vascular tone regulation.
Nevertheless, the role of Orai1- and TRPC1-dependent
SOCC in vascular tone regulation and their possible interaction
with CaV1.2 are still unknown. The current study sought to
characterize the co-activation of SOCC and LTCC upon stimulation
by agonists, and to determine the possible crosstalk
between Orai1, TRPC1, and CaV1.2. Aorta rings and isolated
VSMCobtained from wild type or smooth muscle-selective conditional
CaV1.2 knock-out (CaV1.2KO) mice were used to study
vascular contractility, intracellular Ca2 mobilization, and distribution
of ion channels. We found that serotonin (5-HT) or
store depletion with thapsigargin (TG) enhanced intracellular
free Ca2 concentration ([Ca2 ]i) and stimulated aorta contraction.
These responses were sensitive to LTCC and SOCC inhibitors.
Also, 5-HT- and TG-induced responses were significantly
attenuated in CaV1.2KO mice. Furthermore, hyperpolarization
induced with cromakalim or valinomycin significantly reduced
both 5-HT and TG responses, whereas these responses were
enhanced with LTCC agonist Bay-K-8644. Interestingly, in situ
proximity ligation assay revealed that CaV1.2 interacts with
Orai1 and TRPC1 in untreated VSMC. These interactions
enhanced significantly after stimulation of cells with 5-HT and
TG. Therefore, these data indicate for the first time a functional
interaction between Orai1, TRPC1, and CaV1.2 channels in
VSMC, confirming that upon agonist stimulation, vessel contraction
involves Ca2 entry due to co-activation of Orai1- and
TRPC1-dependent SOCC and LTCC. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2013-45564-C2-1-P | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BFU2013-45564-C2-2-P | es |
dc.description.sponsorship | Instituto de Salud Carlos III RD12/0042/ 0041 | es |
dc.description.sponsorship | Cardiovascular Network “RIC” PI12/00941 | es |
dc.description.sponsorship | Junta de Andalucía PI-0108-2012 | es |
dc.description.sponsorship | Junta de Andalucía P12- CTS-1965 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Biochemistry and Molecular Biology | es |
dc.relation.ispartof | The Journal of Biological Chemistry, 291 (40), 21148-21159. | |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | CaV1.2 | es |
dc.subject | CaV1.2 channel | es |
dc.subject | Orai1 | es |
dc.subject | TRPC1 | es |
dc.subject | Vascular tone regulation | es |
dc.subject | calcium | es |
dc.subject | calcium release-activated calcium channel protein 1 (ORAI1) | es |
dc.subject | excitation-contraction coupling (E-C coupling) | es |
dc.subject | ion channel | es |
dc.subject | vascular smooth muscle cells | es |
dc.title | Orai1 and TRPC1 Proteins Co-localize with CaV1.2 Channels to Form a Signal Complex in Vascular Smooth Muscle Cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica | es |
dc.contributor.group | Universidad de Sevilla. CTS-200: Trasplante Corazon. Conservacion Corazon Donante | es |
idus.format.extent | 12 | es |
dc.journaltitle | The Journal of Biological Chemistry | es |
dc.publication.volumen | 291 | es |
dc.publication.issue | 40 | es |
dc.publication.initialPage | 21148 | es |
dc.publication.endPage | 21159 | es |