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dc.creatorPérez Pérez, María Estheres
dc.creatorLemaire, Stéphane D.es
dc.creatorCrespo González, José Luises
dc.date.accessioned2018-01-05T11:43:29Z
dc.date.available2018-01-05T11:43:29Z
dc.date.issued2016
dc.identifier.citationPérez Pérez, M.E., Lemaire, S.D. y Crespo González, J.L. (2016). Control of autophagy in Chlamydomonas is mediated through redox dependent inactivation of the ATG4 protease. Plant Physiology, 172 (4), 2219-2234.
dc.identifier.issn0032-0889es
dc.identifier.urihttp://hdl.handle.net/11441/68336
dc.description.abstractAutophagy is a major catabolic pathway by which eukaryotic cells deliver unnecessary or damaged cytoplasmic material to the vacuole for its degradation and recycling in order to maintain cellular homeostasis. Control of autophagy has been associated with the production of reactive oxygen species in several organisms including plants and algae but the precise regulatory molecular mechanisms remain unclear. Here, we show that the ATG4 protease, an essential protein for autophagosome biogenesis, plays a central role for the redox regulation of autophagy in the model green alga Chlamydomonas reinhardtii. Our results indicate that the activity of Chlamydomonas ATG4 is regulated by the formation of a single disulfide bond with a low redox potential that can be efficiently reduced by the NADPH/thioredoxin system. Moreover, we found that treatment of Chlamydomonas cells with norflurazon, an inhibitor of carotenoid biosynthesis that generates reactive oxygen species and triggers autophagy in this alga, promotes the oxidation and aggregation of ATG4. We propose that the activity of the ATG4 protease is finely regulated by the intracellular redox state and it is inhibited under stress conditions to ensure lipidation of ATG8 and thus autophagy progression in Chlamydomonas.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society of Plant Biologistses
dc.relation.ispartofPlant Physiology, 172 (4), 2219-2234.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleControl of autophagy in Chlamydomonas is mediated through redox dependent inactivation of the ATG4 proteasees
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/submittedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Moleculares
dc.relation.publisherversionhttp://dx.doi.org/10.1104/pp.16.01582es
dc.identifier.doi10.1104/pp.16.01582es
idus.format.extent15 p.es
dc.journaltitlePlant Physiologyes
dc.publication.volumen172es
dc.publication.issue4es
dc.publication.initialPage2219es
dc.publication.endPage2234es

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