dc.creator | Valladares Ruiz, Ana | es |
dc.creator | Flores García, Enrique | es |
dc.creator | Herrero Moreno, Antonia | es |
dc.date.accessioned | 2018-01-03T15:39:42Z | |
dc.date.available | 2018-01-03T15:39:42Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Valladares Ruiz, A., Flores García, E. y Herrero Moreno, A. (2016). The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation. Molecular Microbiology, 99 (4), 808-819. | |
dc.identifier.issn | 0950-382X | es |
dc.identifier.uri | http://hdl.handle.net/11441/68197 | |
dc.description.abstract | Many filamentous cyanobacteria respond to the external cue of nitrogen scarcity by the differentiation of heterocysts, cells specialized in the fixation of atmospheric nitrogen in oxic environments. Heterocysts follow a spatial pattern along the filament of two heterocysts separated by ca. 10–15 vegetative cells performing oxygenic photosynthesis. HetR is a transcriptional regulator that directs heterocyst differentiation. In the model strain Anabaena sp. PCC 7120, the HetR protein was observed in various oligomeric forms in vivo, including a tetramer that peaked with maximal hetR expression during differentiation. Tetramers were not detected in a hetR point mutant incapable of differentiation, but were conspicuous in an over-differentiating strain lacking the PatS inhibitor. In differentiated filaments the HetR tetramer was restricted to heterocysts, being undetectable in vegetative cells. HetR co-purified with RNA polymerase from Anabaena mainly as a tetramer. In vitro, purified recombinant HetR was distributed between monomers, dimers, trimers and tetramers, and it was phosphorylated when incubated with (γ-32P)ATP. Phosphorylation and PatS hampered the accumulation of HetR tetramers and impaired HetR binding to DNA. In summary, tetrameric HetR appears to represent a functionally relevant form of HetR, whose abundance in the Anabaena filament could be negatively regulated by phosphorylation and by PatS. | es |
dc.description.sponsorship | España, Gobierno BFU2010-17980 and BFU2013-44686-P | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Wiley | es |
dc.relation.ispartof | Molecular Microbiology, 99 (4), 808-819. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Heterocyst differentiation | es |
dc.subject | Post-translational modification | es |
dc.subject | Anabaena | es |
dc.title | The heterocyst differentiation transcriptional regulator HetR of the filamentous cyanobactrium Anabaena forms tetramers and can be regulated by phosphorylation | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/submittedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | BFU2010-17980 | es |
dc.relation.projectID | BFU2013-44686-P | es |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1111/mmi.13268/epdf | es |
dc.identifier.doi | doi: 10.1111/mmi.13268 | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Molecular Microbiology | es |
dc.publication.volumen | 99 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 808 | es |
dc.publication.endPage | 819 | es |
dc.contributor.funder | Gobierno de España | |