dc.creator | Ojeda Servián, Valle | es |
dc.creator | Pérez Ruiz, Juan Manuel | es |
dc.creator | González García, María de la Cruz | es |
dc.creator | Armario Nájera, María Victoria | es |
dc.creator | Sahrawy, Mariam | es |
dc.creator | Serrato, Antonio J. | es |
dc.creator | Cejudo Fernández, Francisco Javier | es |
dc.date.accessioned | 2017-12-18T18:10:16Z | |
dc.date.available | 2017-12-18T18:10:16Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Ojeda Servián, V., Pérez Ruiz, J.M., González García, M.d.l.C., Armario Nájera, M.V., Sahrawy, M., Serrato, A.J. y Cejudo Fernández, F.J. (2017). NADPH Thioredoxin Reductase C and Thioredoxins Act Concertedly in Seedling Development. Plant Physiology, 174, 1436-1448. | |
dc.identifier.issn | 0032-0889 | es |
dc.identifier.uri | http://hdl.handle.net/11441/67756 | |
dc.description.abstract | Thiol-dependent redox regulation of enzyme activity plays a central role in the rapid acclimation of chloroplast metabolism to
ever-fluctuating light availability. This regulatory mechanism relies on ferredoxin reduced by the photosynthetic electron
transport chain, which fuels reducing power to thioredoxins (Trxs) via a ferredoxin-dependent Trx reductase. In addition,
chloroplasts harbor an NADPH-dependent Trx reductase, which has a joint Trx domain at the carboxyl terminus, termed NTRC.
Thus, a relevant issue concerning chloroplast function is to establish the relationship between these two redox systems and its
impact on plant development. To address this issue, we generated Arabidopsis (Arabidopsis thaliana) mutants combining the
deficiency of NTRC with those of Trxs f, which participate in metabolic redox regulation, and that of Trx x, which has
antioxidant function. The ntrc-trxf1f2 and, to a lower extent, ntrc-trxx mutants showed severe growth-retarded phenotypes,
decreased photosynthesis performance, and almost abolished light-dependent reduction of fructose-1,6-bisphosphatase.
Moreover, the combined deficiency of both redox systems provokes aberrant chloroplast ultrastructure. Remarkably, both the
ntrc-trxf1f2 and ntrc-trxx mutants showed high mortality at the seedling stage, which was overcome by the addition of an
exogenous carbon source. Based on these results, we propose that NTRC plays a pivotal role in chloroplast redox regulation,
being necessary for the activity of diverse Trxs with unrelated functions. The interaction between the two thiol redox systems is
indispensable to sustain photosynthesis performed by cotyledons chloroplasts, which is essential for early plant development. | es |
dc.description.sponsorship | España Mineco BIO2013-43556-P | es |
dc.description.sponsorship | España, Junta de Andalucía CVI-5919 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society of Plant Biologists | es |
dc.relation.ispartof | Plant Physiology, 174, 1436-1448. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | NADPH Thioredoxin Reductase C and Thioredoxins Act Concertedly in Seedling Development | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | |
dc.relation.projectID | BIO2013-43556-P | es |
dc.relation.projectID | CVI-5919 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1104/pp.17.00481 | es |
dc.identifier.doi | 10.1104/pp.17.00481 | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | Plant Physiology | es |
dc.publication.volumen | 174 | es |
dc.publication.initialPage | 1436 | es |
dc.publication.endPage | 1448 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | |
dc.contributor.funder | Junta de Andalucía | |