dc.creator | Monreal Hermoso, José Antonio | es |
dc.creator | López Baena, Francisco Javier | es |
dc.creator | Vidal, Jean | es |
dc.creator | Echevarría Ruiz de Vargas, Cristina | es |
dc.creator | García-Mauriño Ruiz-Berdejo, Sofía | es |
dc.date.accessioned | 2017-12-04T15:26:46Z | |
dc.date.available | 2017-12-04T15:26:46Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Monreal Hermoso, J.A., López Baena, F.J., Vidal, J., Echevarría Ruiz de Vargas, C. y García-Mauriño Ruiz-Berdejo, S. (2010). Involvement of phospholipase D and phosphatidic acid in the light-dependent up-regulation of sorghum leaf phosphoenolpyruvate carboxylase-kinase. Journal of Experimental Botany, 61 (10), 2819-2827. | |
dc.identifier.issn | 0022-0957 (impreso) | es |
dc.identifier.issn | 1460-2431 (electrónico) | es |
dc.identifier.uri | http://hdl.handle.net/11441/67223 | |
dc.description.abstract | The photosynthetic phosphoenolpyruvate carboxylase (C4-PEPC) is regulated by phosphorylation by a phosphoenolpyruvate carboxylase kinase (PEPC-k). In Digitaria sanguinalis mesophyll protoplasts, this light-mediated transduction cascade principally requires a phosphoinositide-specific phospholipase C (PI-PLC) and a Ca2+-dependent step. The present study investigates the cascade components at the higher integrated level of Sorghum bicolor leaf discs and leaves. PEPC-k up-regulation required light and photosynthetic electron transport. However, the PI-PLC inhibitor U-73122 and inhibitors of calcium release from intracellular stores only partially blocked this process. Analysis of [32P]phosphate-labelled phospholipids showed a light-dependent increase in phospholipase D (PLD) activity. Treatment of leaf discs with n-butanol, which decreases the formation of phosphatidic acid (PA) by PLD, led to the partial inhibition of the C4-PEPC phosphorylation, suggesting the participation of PLD/PA in the signalling cascade. PPCK1 gene expression was strictly light-dependent. Addition of neomycin or n-butanol decreased, and a combination of both inhibitors markedly reduced PPCK1 expression and the concomitant rise in PEPC-k activity. The calcium/calmodulin antagonist W7 blocked the light-dependent up-regulation of PEPC-k, pointing to a Ca2+-dependent protein kinase (CDPK) integrating both second messengers, calcium and PA, which were shown to increase the activity of sorghum CDPK. | es |
dc.description.sponsorship | Junta de Andalucía PAI group BIO298 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Journal of Experimental Botany, 61 (10), 2819-2827. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Ca2+-dependent protein kinase | es |
dc.subject | phosphatidic acid | es |
dc.subject | phosphoenolpyruvate carboxylase | es |
dc.subject | phosphoenolpyruvate carboxylase kinase | es |
dc.subject | phospholipase C | es |
dc.subject | phospholipase D | es |
dc.subject | Sorghum bicolor | es |
dc.title | Involvement of phospholipase D and phosphatidic acid in the light-dependent up-regulation of sorghum leaf phosphoenolpyruvate carboxylase-kinase | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | BIO298 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1093/jxb/erq114 | es |
dc.identifier.doi | 10.1093/jxb/erq114 | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Journal of Experimental Botany | es |
dc.publication.volumen | 61 | es |
dc.publication.issue | 10 | es |
dc.publication.initialPage | 2819 | es |
dc.publication.endPage | 2827 | es |
dc.contributor.funder | Junta de Andalucía | |