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dc.creatorSein-Echaluce, Violeta C.es
dc.creatorGonzález, Andréses
dc.creatorNapolitano, Mauroes
dc.creatorLuque, Ignacioes
dc.creatorBarja, Franciscoes
dc.creatorPeleato, M.L.es
dc.creatorFillat, M.F.es
dc.date.accessioned2017-10-31T17:28:24Z
dc.date.available2017-10-31T17:28:24Z
dc.date.issued2015
dc.identifier.citationSein-Echaluce, V.C., González, A., Napolitano, M., Luque, I., Barja, F., Peleato, M.L. y Fillat, M.F. (2015). Zur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120. Environmental Microbiology, 17 (6), 2006-2017.
dc.identifier.issn1462-2912 (impreso)es
dc.identifier.issn1462-2920 (electrónico)es
dc.identifier.urihttp://hdl.handle.net/11441/65582
dc.description.abstractIron and zinc are necessary nutrients whose homeostasis is tightly controlled by members of the ferric uptake regulator (FUR) superfamily in the cyanobacterium Anabaena sp. PCC7120. Although the link between iron metabolism and oxidative stress management is well documented, little is known about the connection between zinc homeostasis and the oxidative stress response in cyanobacteria. Zinc homeostasis in Anabaena is controlled by Zur, also named FurB. When overexpressed in Escherichia coli, Zur (FurB) improved cell survival during oxidative stress. In order to investigate the possible correlation between Zur and the oxidative stress response in Anabaena, zur deletion and zur-overexpressing strains have been constructed, and the consequences of Zur imbalance evaluated. The lack of Zur increased sensitivity to hydrogen peroxide (H2 O2 ), whereas an excess of Zur enhanced oxidative stress resistance. Both mutants displayed pleiotropic phenotypes, including alterations on the filament surfaces observable by scanning electron microscopy, reduced content of endogenous H2 O2 and altered expression of sodA, catalases and several peroxiredoxins. Transcriptional and biochemical analyses unveiled that the appropriate level of Zur is required for proper control of the oxidative stress response and allowed us to identify major antioxidant enzymes as novel members of the Zur regulon.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherWiley-Blackwelles
dc.relation.ispartofEnvironmental Microbiology, 17 (6), 2006-2017.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectBacteriaes
dc.subjectEcophysiologyes
dc.subjectEnvironmental signal/stress responseses
dc.subjectGene expression/regulationes
dc.subjectMetabolic networkses
dc.subjectMetalses
dc.subjectMicrobial geneticses
dc.titleZur (FurB) is a key factor in the control of the oxidative stress response in Anabaena sp. PCC 7120es
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/acceptedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.publisherversionhttp://dx.doi.org/10.1111/1462-2920.12628es
dc.identifier.doi10.1111/1462-2920.12628es
idus.format.extent42 p.es
dc.journaltitleEnvironmental Microbiologyes
dc.publication.volumen17es
dc.publication.issue6es
dc.publication.initialPage2006es
dc.publication.endPage2017es

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