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dc.creatorPérez Martín, Martaes
dc.creatorBably-Haas,Crysten E.es
dc.creatorPérez Pérez, Maria Estheres
dc.creatorAndrés Garrido, Ascensiónes
dc.creatorBlaby, Ian K.es
dc.creatorMerchant, Sabeeha S.es
dc.creatorCrespo, José Luises
dc.date.accessioned2017-10-31T15:34:03Z
dc.date.available2017-10-31T15:34:03Z
dc.date.issued2015
dc.identifier.citationPérez Martín, M., Bably-Haas, r.E., Pérez Pérez, M.E., Andrés Garrido, A., Blaby, I.K., Merchant, S.S. y Crespo, J.L. (2015). Activation of Autophagy by Metals in Chlamydomonas reinhardtii. Eukaryotic Cell, 14 (9), 964-973.
dc.identifier.issn1535-9778 (impreso)es
dc.identifier.issn1535-9786 (electrónico)es
dc.identifier.urihttp://hdl.handle.net/11441/65576
dc.description.abstractAutophagy is an intracellular self-degradation pathway by which eukaryotic cells recycle their own material in response to specific stress conditions. Exposure to high concentrations of metals causes cell damage, although the effect of metal stress on autophagy has not been explored in photosynthetic organisms. In this study, we investigated the effect of metal excess on autophagy in the model unicellular green alga Chlamydomonas reinhardtii. We show in cells treated with nickel an upregulation of ATG8 that is independent of CRR1, a global regulator of copper signaling in Chlamydomonas. A similar effect on ATG8 was observed with copper and cobalt but not with cadmium or mercury ions. Transcriptome sequencing data revealed an increase in the abundance of the protein degradation machinery, including that responsible for autophagy, and a substantial overlap of that increased abundance with the hydrogen peroxide response in cells treated with nickel ions. Thus, our results indicate that metal stress triggers autophagy in Chlamydomonas and suggest that excess nickel may cause oxidative damage, which in turn activates degradative pathways, including autophagy, to clear impaired components and recover cellular homeostasises
dc.description.sponsorshipMinisterio de Economía y Competitividad BFU2012-35913es
dc.description.sponsorshipJunta de Andalucía CVI-7336es
dc.description.sponsorshipNational Institutes of Health GM42143, R24 GM092473es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherASM Internationales
dc.relation.ispartofEukaryotic Cell, 14 (9), 964-973.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleActivation of Autophagy by Metals in Chlamydomonas reinhardtiies
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.relation.projectIDinfo:eu-repo/grantAgreement/MINECO/BFU2012-35913es
dc.relation.projectIDCVI-7336es
dc.relation.projectIDGM42143es
dc.relation.projectIDR24 GM092473es
dc.relation.publisherversionhttp://dx.doi.org/10.1128/EC.00081-15es
dc.identifier.doi10.1128/EC.00081-15es
idus.format.extent10 p.es
dc.journaltitleEukaryotic Celles
dc.publication.volumen14es
dc.publication.issue9es
dc.publication.initialPage964es
dc.publication.endPage973es

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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