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dc.creatorPrado Velasco, José Félixes
dc.creatorAguilera López, Andréses
dc.date.accessioned2017-07-05T14:17:17Z
dc.date.available2017-07-05T14:17:17Z
dc.date.issued2005
dc.identifier.citationPrado Velasco, J.F. y Aguilera López, A. (2005). Partial depletion of histone H4 increases homologous recombination-mediated genetic instability. Molecular and Cellular Biology, 25 (4), 1526-1536.
dc.identifier.issn0270-7306es
dc.identifier.urihttp://hdl.handle.net/11441/62056
dc.description.abstractDNA replication can be a source of genetic instability. Given the tight connection between DNA replication and nucleosome assembly, we analyzed the effect of a partial depletion of histone H4 on genetic instability mediated by homologous recombination. A Saccharomyces cerevisiae strain was constructed in which the expression of histone H4 was driven by the regulated tet promoter. In agreement with defective nucleosome assembly, partial depletion of histone H4 led to subtle changes in plasmid superhelical density and chromatin sensitivity to micrococcal nuclease. Under these conditions, homologous recombination between ectopic DNA sequences was increased 20-fold above the wild-type levels. This hyperrecombination was not associated with either defective repair or transcription but with an accumulation of recombinogenic DNA lesions during the S and G2/M phases, as determined by an increase in the proportion of budded cells containing Rad52-yellow fluorescent protein foci. Consistently, partial depletion of histone H4 caused a delay during the S and G2/M phases. Our results suggest that histone deposition defects lead to the formation of recombinogenic DNA structures during replication that increase genomic instability.es
dc.description.sponsorshipMinisterio de Ciencia y Tecnología BMC2000-0439 SAF2003-00204es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherAmerican Society for Microbiologyes
dc.relation.ispartofMolecular and Cellular Biology, 25 (4), 1526-1536.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titlePartial depletion of histone H4 increases homologous recombination-mediated genetic instabilityes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.projectIDBMC2000-0439es
dc.relation.projectIDSAF2003-00204es
dc.relation.publisherversionhttp://dx.doi.org/10.1128/MCB.25.4.1526–1536.2005es
dc.identifier.doi10.1128/MCB.25.4.1526–1536.2005es
idus.format.extent11 p.es
dc.journaltitleMolecular and Cellular Biologyes
dc.publication.volumen25es
dc.publication.issue4es
dc.publication.initialPage1526es
dc.publication.endPage1536es
dc.identifier.sisius6655174es
dc.contributor.funderMinisterio de Ciencia y Tecnología (MCYT). España

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