Artículo
Neddylation inhibits CtIP-mediated resection and regulates DNA double strand break repair pathway choice
Autor/es | Jimeno González, Sonia
Fernández Ávila, María Jesús Cruz García, Andrés Cepeda García, Cristina Gómez Cabello, Daniel Huertas Sánchez, Pablo |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2015 |
Fecha de depósito | 2017-03-17 |
Publicado en |
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Resumen | DNA double strand breaks are the most cytotoxic lesions that can occur on the DNA. They can be repaired by different mechanisms and optimal survival requires a tight control between them. Here we uncover protein deneddylation ... DNA double strand breaks are the most cytotoxic lesions that can occur on the DNA. They can be repaired by different mechanisms and optimal survival requires a tight control between them. Here we uncover protein deneddylation as a major controller of repair pathway choice. Neddylation inhibition changes the normal repair profile toward an increase on homologous recombination. Indeed, RNF111/UBE2M-mediated neddylation acts as an inhibitor of BRCA1 and CtIP-mediated DNA end resection, a key process in repair pathway choice. By controlling the length of ssDNA produced during DNA resection, protein neddylation not only affects the choice between NHEJ and homologous recombination but also controls the balance between different recombination subpathways. Thus, protein neddylation status has a great impact in the way cells respond to DNA breaks. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España |
Identificador del proyecto | info:eu-repo/grantAgreement/MINECO/SAF2010-14877 |
Cita | Jimeno González, S., Fernández Ávila, M.J., Cruz García, A., Cepeda García, C., Gómez Cabello, D. y Huertas Sánchez, P. (2015). Neddylation inhibits CtIP-mediated resection and regulates DNA double strand break repair pathway choice. Nucleic Acids Research, 43 (2), 987-999. |
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