Artículo
DNA polymerase λ, a novel DNA repair enzyme in human cells
Autor/es | García Díaz, Miguel
Bebenek, Katarzyna Sabariegos, Rosario Domínguez, Orlando Rodríguez, Josana Renato Kirchhoff, Tomas García Palomero, Esther Picher, Ángel J. Juárez, Raquel Ruiz Pérez, José Francisco Kunkel, Thomas A. Blanco, Luis |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2002 |
Fecha de depósito | 2017-02-15 |
Publicado en |
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Resumen | DNA polymerase lambda (pol λ) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5′-deoxyribose-5-phosphate lyase ... DNA polymerase lambda (pol λ) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5′-deoxyribose-5-phosphate lyase activity in pol A supports a function of this enzyme in base excision repair. However, the biochemical properties of the polymerization activity of this enzyme are still largely unknown. We have cloned and purified human pol A to homogeneity in a soluble and active form, and we present here a biochemical description of its polymerization features. In support of a role in DNA repair, pol λ inserts nucleotides in a DNA template-dependent manner and is processive in small gaps containing a 5′-phosphate group. These properties, together with its nucleotide insertion fidelity parameters and lack of proofreading activity, indicate that pol A is a novel β-like DNA polymerase. However, the high affinity of pol λ for dNTPs (37-fold over pol β) is consistent with its possible involvement in DNA transactions occurring under low cellular levels of dNTPs. This suggests that, despite their similarities, pol β and pol λ have nonredundant in vivo functions |
Cita | García Díaz, M., Bebenek, K., Sabariegos, R., Domínguez, O., Rodríguez, J.R., Kirchhoff, T.,...,Blanco, L. (2002). DNA polymerase λ, a novel DNA repair enzyme in human cells. Journal of Biological Chemistry, 277 (15), 13184-13191. |
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