Artículo
DNA polymerase λ, a novel DNA repair enzyme in human cells
Autor/es | García Díaz, Miguel
Bebenek, Katarzyna Sabariegos, Rosario Domínguez, Orlando Rodríguez, Josana Renato Kirchhoff, Tomas García Palomero, Esther Picher, Ángel J. Juárez, Raquel Ruiz Pérez, José Francisco ![]() ![]() ![]() ![]() ![]() ![]() ![]() Kunkel, Thomas A. Blanco, Luis |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2002 |
Fecha de depósito | 2017-02-15 |
Publicado en |
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Resumen | DNA polymerase lambda (pol λ) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5′-deoxyribose-5-phosphate lyase ... DNA polymerase lambda (pol λ) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5′-deoxyribose-5-phosphate lyase activity in pol A supports a function of this enzyme in base excision repair. However, the biochemical properties of the polymerization activity of this enzyme are still largely unknown. We have cloned and purified human pol A to homogeneity in a soluble and active form, and we present here a biochemical description of its polymerization features. In support of a role in DNA repair, pol λ inserts nucleotides in a DNA template-dependent manner and is processive in small gaps containing a 5′-phosphate group. These properties, together with its nucleotide insertion fidelity parameters and lack of proofreading activity, indicate that pol A is a novel β-like DNA polymerase. However, the high affinity of pol λ for dNTPs (37-fold over pol β) is consistent with its possible involvement in DNA transactions occurring under low cellular levels of dNTPs. This suggests that, despite their similarities, pol β and pol λ have nonredundant in vivo functions |
Cita | García Díaz, M., Bebenek, K., Sabariegos, R., Domínguez, O., Rodríguez, J.R., Kirchhoff, T.,...,Blanco, L. (2002). DNA polymerase λ, a novel DNA repair enzyme in human cells. Journal of Biological Chemistry, 277 (15), 13184-13191. |
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