dc.creator | Comino Montilla, Isabel María | es |
dc.creator | Bernardo, David | es |
dc.creator | Bancel, Emmanuelle | es |
dc.creator | Moreno Amador, María de Lourdes | es |
dc.creator | Sánchez, Borja | es |
dc.creator | Barro, Francisco | es |
dc.creator | Šuligoj, Tanja | es |
dc.creator | Ciclitira, Paul Jonathan | es |
dc.creator | Cebolla, Ángel | es |
dc.creator | Knight, Stella Catherine | es |
dc.creator | Branlard, Gérard | es |
dc.creator | Sousa Martín, Carolina | es |
dc.date.accessioned | 2016-11-21T12:42:38Z | |
dc.date.available | 2016-11-21T12:42:38Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Comino Montilla, I.M., Bernardo, D., Bancel, E., Moreno Amador, M.d.L., Sánchez, B., Barro, F.,...,Sousa Martín, C. (2016). Identification and molecular characterization of oat peptides implicated on coeliac immune response. Food and Nutrition Research, 60, 1-13. | |
dc.identifier.issn | 1654-6628 | es |
dc.identifier.uri | http://hdl.handle.net/11441/48937 | |
dc.description.abstract | Background
: Oats provide important nutritional and pharmacological properties, although their safety in
coeliac patients remains controversial. Previous studies have confirmed that the reactivity of the anti-33-mer
monoclonal antibody with different oat varieties is proportional to the immune responses in terms of T-cell
proliferation. Although the impact of these varieties on the adaptive response has been studied, the role of the
dendritic cells (DC) is still poorly understood. The aim of this study is to characterize different oat fractions
and to study their effect on DC from coeliac patients.
Methods and results
: Protein fractions were isolated from oat grains and analyzed by SDS
PAGE. Several
proteins were characterized in the prolamin fraction using immunological and proteomic tools, and by
Nano-
LC-MS/MS
. These proteins, analogous to
a
- and
g
-gliadin-like, showed reactive sequences to anti-33-mer
antibody suggesting their immunogenic potential. That was further confirmed as some of the newly identified
oat peptides had a differential stimulatory capacity on circulating DC from coeliac patients compared with
healthy controls.
Conclusions
: This is the first time, to our knowledge, where newly identified oat peptides have been shown to
elicit a differential stimulatory capacity on circulating DC obtained from coeliac patients, potentially
identifying immunogenic properties of these oat peptides | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Co-Action Publishing | es |
dc.relation.ispartof | Food and Nutrition Research, 60, 1-13. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Coeliac disease | es |
dc.subject | Oats | es |
dc.subject | Immune response | es |
dc.subject | Gluten-free diet | es |
dc.title | Identification and molecular characterization of oat peptides implicated on coeliac immune response | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | http://www.foodandnutritionresearch.net/index.php/fnr/article/view/30324/44772 | es |
idus.format.extent | 13 p. | es |
dc.journaltitle | Food and Nutrition Research | es |
dc.publication.volumen | 60 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 13 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/48937 | |