dc.creator | Delgado, María Jesús | es |
dc.creator | Yeoman, Kay H. | es |
dc.creator | Wu, Guanghui | es |
dc.creator | Vargas Macías, Carmen | es |
dc.creator | Davies, Andrea E. | es |
dc.creator | Poole, Robert K. | es |
dc.creator | Johnston, Andrew W. B. | es |
dc.creator | Downi, J. Allan | es |
dc.date.accessioned | 2016-06-22T09:34:35Z | |
dc.date.available | 2016-06-22T09:34:35Z | |
dc.date.issued | 1995 | |
dc.identifier.citation | Delgado, M.J., Yeoman, K.H., Wu, G., Vargas, C., Davies, A.E., Poole, R.K.,...,Downi, J.A. (1995). Characterization of the cycHJKL genes involved in cytochrome c biogenesis and symbiotic nitrogen fixation in rhizobium leguminosarum. Journal of Bacteriology, 177 (17), 4927-4934. | |
dc.identifier.issn | 0021-9193 | es |
dc.identifier.uri | http://hdl.handle.net/11441/42609 | |
dc.description.abstract | Mutants of Rhizobium leguminosarum bv. viciae unable to respire via the cytochrome aa3 pathway were
identified by the inability to oxidize N,N*-dimethyl-p-phenylenediamine. Two mutants which were complemented
by cosmid pIJ1942 from an R. leguminosarum clone bank were identified. Although pea nodules induced
by these mutants contained many bacteroids, no symbiotic nitrogen fixation was detected. Heme staining of
cellular proteins revealed that all cytochrome c-type heme proteins were absent. These mutants lacked
spectroscopically detectable cytochrome c, but cytochromes aa3 and d were present, the latter at a higherthan-
normal level. DNA sequence analysis of complementing plasmids revealed four apparently cotranscribed
open reading frames (cycH, cycJ, cycK, and cycL). CycH, CycJ, CycK, and CycL are homologous to Bradyrhizobium
japonicum and Rhizobium meliloti proteins thought to be involved in the attachment of heme to
cytochrome c apoproteins; CycK and CycL are also homologous to the Rhodobacter capsulatus ccl1 and ccl2 gene
products and the Escherichia coli nrfE and nrfF gene products involved in the assembly of c-type cytochromes.
The absence of cytochrome c heme proteins in these R. leguminosarum mutants is consistent with the view that
the cycHJKL operon could be involved in the attachment of heme to apocytochrome c | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Journal of Bacteriology, 177 (17), 4927-4934. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Characterization of the cycHJKL genes involved in cytochrome c biogenesis and symbiotic nitrogen fixation in rhizobium leguminosarum | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC177267/ | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Journal of Bacteriology | es |
dc.publication.volumen | 177 | es |
dc.publication.issue | 17 | es |
dc.publication.initialPage | 4927 | es |
dc.publication.endPage | 4934 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/42609 | |