dc.creator | Torres, María J. | es |
dc.creator | Argandoña Bertrán, Montserrat | es |
dc.creator | Vargas Macías, Carmen | es |
dc.creator | Bedmar, Eulogio José | es |
dc.creator | Fischer, Hans Martin | es |
dc.creator | Mesa, Socorro | es |
dc.creator | Delgado, María Jesús | es |
dc.date.accessioned | 2016-06-20T09:55:15Z | |
dc.date.available | 2016-06-20T09:55:15Z | |
dc.date.issued | 2014 | |
dc.identifier.citation | Torres, M.J., Argandoña Bertrán, M., Vargas, C., Bedmar, E.J., Fischer, H.M., Mesa, S. y Delgado, M.J. (2014). The global response regulator RegR controls expression of denitrification genes in Bradyrhizobium japonicum. PLoS One, 9 (6), 1-11. | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | http://hdl.handle.net/11441/42461 | |
dc.description.abstract | Bradyrhizobium japonicum
RegSR regulatory proteins belong to the family of two-component regulatory systems, and
orthologs are present in many Proteobacteria where they globally control gene expression mostly in a redox-responsive
manner. In this work, we have performed a transcriptional profiling of wild-type and
regR
mutant cells grown under anoxic
denitrifying conditions. The comparative analyses of wild-type and
regR
strains revealed that almost 620 genes induced in
the wild type under denitrifying conditions were regulated (directly or indirectly) by RegR, pointing out the important role
of this protein as a global regulator of denitrification. Genes controlled by RegR included
nor
and
nos
structural genes
encoding nitric oxide and nitrous oxide reductase, respectively, genes encoding electron transport proteins such as
cycA
(blr7544) or
cy
2
(bll2388), and genes involved in nitric oxide detoxification (blr2806-09) and copper homeostasis (
copCAB
), as
well as two regulatory genes (bll3466, bll4130). Purified RegR interacted with the promoters of
norC
(blr3214),
nosR
(blr0314), a
fixK
-like gene (bll3466), and bll4130, which encodes a LysR-type regulator. By using fluorescently labeled
oligonucleotide extension (FLOE), we were able to identify two transcriptional start sites located at about 35 (P1) and 22 (P2)
bp upstream of the putative translational start codon of
norC
. P1 matched with the previously mapped 5
9
end of
norC
mRNA
which we demonstrate in this work to be under FixK
2
control. P2 is a start site modulated by RegR and specific for anoxic
conditions. Moreover, qRT-PCR experiments, expression studies with a
norC-lacZ
fusion, and heme
c
-staining analyses
revealed that anoxia and nitrate are required for RegR-dependent induction of
nor
genes, and that this control is
independent of the sensor protein RegS | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS One, 9 (6), 1-11. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The global response regulator RegR controls expression of denitrification genes in Bradyrhizobium japonicum | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | 10.1371/journal.pone.0099011 | es |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0099011 | es |
idus.format.extent | 12 p. | es |
dc.journaltitle | PLoS One | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 6 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 11 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/42461 | |