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dc.creatorVelasco López, Ángeles
dc.creatorHendricks, Lindaes
dc.creatorMoremen, Kelly
dc.creatorTulsiani, D. R.
dc.creatorTouster, Oscares
dc.creatorFarquhar, Marilyn Gistes
dc.identifier.citationVelasco López, Á., Hendricks, L., Moremen, .W., Tulsiani, D.R.P., Touster, . y Farquhar, M.G. (1993). Cell type-dependent variations in the subcellular distribution of alpha-mannosidase I and II. Journal of Cell Biology, 122(1):39-51
dc.description.abstractalpha-mannosidases I and II (Man I and II) are resident enzymes of the Golgi complex involved in oligosaccharide processing during N-linked glycoprotein biosynthesis that are widely considered to be markers of the cis- and medial-Golgi compartments, respectively. We have investigated the distribution of these enzymes in several cell types by immunofluorescence and immunoelectron microscopy. Man II was most commonly found in medial- and/or trans- cisternae but showed cell type-dependent variations in intra-Golgi distribution. It was variously localized to either medial (NRK and CHO cells), both medial and trans (pancreatic acinar cells, enterocytes), or trans- (goblet cells) cisternae, or distributed across the entire Golgi stack (hepatocytes and some enterocytes). The distribution of Man I largely coincided with that of Man II in that it was detected primarily in medial- and trans-cisternae. It also showed cell type dependent variations in its intra-Golgi distribution. Man I and Man II were also detected within secretory granules and at the cell surface of some cell types (enterocytes, pancreatic acinar cells, goblet cells). In the case of Man II, cell surface staining was shown not to be due to antibody cross-reactivity with oligosaccharide epitopes. These results indicate that the distribution of Man I and Man II within the Golgi stack of a given cell type overlaps considerably, and their distribution from one cell type to another is more variable and less compartmentalized than previously
dc.publisherRockefeller University Presses
dc.relation.ispartofJournal of Cell Biology, 122(1):39-51
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.titleCell type-dependent variations in the subcellular distribution of alpha-mannosidase I and IIes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Celulares

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
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