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dc.creatorEspartero Sánchez, José Luis
dc.creatorGallardo, Elena
dc.creatorMadrona, Andrés
dc.creatorPalma Valdés, Rocío
dc.creatorTrujillo, Mariana
dc.creatorSantiago Pavón, Martiniano
dc.description.abstractHydroxytyrosol is a well-known phenolic compound with antioxidant properties that is found in virgin olive oil. Studies have shown that virgin olive oil has neuroprotective effects in rats; thus the purpose of the present study was to investigate the neuroprotective effect of hydroxytyrosol in rats. Additionally, this study aimed to investigate the neuroprotective potential of a homologous series of compounds with better lipophilic profiles in order to increase the assortment of compounds with a putative effect against Parkinson's disease (PD). In this context, the inhibition of catechol-O-methyl transferase (COMT) activity by hydroxytyrosol, nitrohydroxytyrosol, nitrohydroxytyrosol acetate and ethyl nitrohydroxytyrosol ether was investigated by measuring intracellular dopamine and its metabolite levels in the corpus striatum by high performance liquid chromatography (HPLC) with electrochemical detection. The animals received an acute (single dose; 20 mg kg-1; i.p.) or chronic (one daily dose for 5 days; 20 mg kg-1; i.p.) treatment of hydroxytyrosol and its nitroderivatives. For comparison, a commercial COMT inhibitor, Ro 41-0960, was also included. Our data show that acute and chronic systemic administration of these compounds produced a clear and statistically significant increase in the intracellular levels of dopamine and its metabolite, 3,4-dihydroxyphenylacetic acid. The increase in dopamine levels was very similar to the increase seen with Ro 41-0960 treatment. The effect of chronic treatment was stronger than that of acute treatment. With respect to the intracellular level of homovanillic acid, Ro 41-0960 produced a statistically significant decrease which it was not observed when hydroxytyrosol and its nitroderivatives were systemically administered. However, the chronic homovanillic acid treatment effect was stronger than the acute treatment. The results suggest that these compounds could inhibit COMT
dc.publisherRoyal Society of Chemistryes
dc.relation.ispartofRSC Advances, 4, 61086-61091es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.subjectChemical detectiones
dc.subjectHigh performance liquid chromatographyes
dc.subjectOil shalees
dc.subjectOil wellses
dc.subjectOlive oiles
dc.subjectRats 3,4-dihydroxyphenylacetic acidses
dc.subjectAntioxidant propertieses
dc.subjectCatechol-o-methyl transferasees
dc.subjectELectrochemical detectiones
dc.subjectIntracellular levelses
dc.subjectNeuroprotective effectses
dc.subjectParkinson's diseasees
dc.subjectSystemic administrationes
dc.titleThe effect of hydroxytyrosol and its nitroderivatives on catechol-O-methyl transferase activity in rat striatal tissuees
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Química Orgánica y Farmacéuticaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Bioquímica y Biología Moleculares

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional