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dc.creatorGarcía-Mauriño, Sofía
dc.creatorEchevarría Ruiz de Vargas, Cristina
dc.creatorVargas Muñoz, María de los Ángeles
dc.creatorAparicio, Pedro J.
dc.creatorMaldonado Ruiz, José María
dc.date.accessioned2016-02-15T12:13:07Z
dc.date.available2016-02-15T12:13:07Z
dc.date.issued1984
dc.identifier.issn1865-7125es
dc.identifier.urihttp://hdl.handle.net/11441/34763
dc.description.abstractNitrate reductase from spinach (Spinacia oleracea L.) leaves, which had been inactivated in vitro by incubation with N A D H and cyanide, was fully reactivated in minutes when irradiated in anaerobic conditions with red light in the presence o f m ethylene blue. Both the rate and the extent of reactivation increased with light intensity ( 6 to 100 W - m “ 2) and dye concentration (1 to 10 jiM). On the contrary, photoreactivation was com pletely abolished when N A D H or ethylenediaminetetra-acetic acid were present during irradiation. W e propose that methylene blue, when photo excited, exhibits a redox potential positive enough to reoxidise the CN~-reduced molybdenum complex settled in the inactive enzyme, thus causing its reactivation. On the other hand, prolonged irradiation o f nitrate reductase, under air and in the presence o f methylene blue, promoted an oxygen-dependent irreversible inactivation o f the two partial activities of the enzyme. This inactivation was markedly enhanced in 77% deuterated water and greatly prevented by azide, which indicates that singlet oxygen is the species primarily involved in the photooxidative inactivation o f the enzymees
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherDe Gruyteres
dc.relation.ispartofZeitschrift für Naturforschung - Section C Journal of Biosciences,39C, 1079-1094es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMethylene Bluees
dc.subjectNitrate Reductasees
dc.subjectPhotosensitizationes
dc.subjectSinglet Oxygenes
dc.subjectSpinaciaes
dc.titleRed-light effects sensitized by methylene blue on nitrate reductase from spinach (Spinacia Oleracea L.) leaveses
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Vegetal y Ecologíaes
dc.relation.publisherversionhttp://zfn.mpdl.mpg.de/data/Reihe_C/39/ZNC-1984-39c-1079.pdfes
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/34763

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Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Except where otherwise noted, this item's license is described as: Attribution-NonCommercial-NoDerivatives 4.0 Internacional