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Artículo
Red-light effects sensitized by methylene blue on nitrate reductase from spinach (Spinacia Oleracea L.) leaves
dc.creator | García-Mauriño Ruiz-Berdejo, Sofía | |
dc.creator | Echevarría Ruiz de Vargas, Cristina | |
dc.creator | Vargas Muñoz, María de los Ángeles | |
dc.creator | Aparicio, Pedro J. | |
dc.creator | Maldonado Ruiz, José María | |
dc.date.accessioned | 2016-02-15T12:13:07Z | |
dc.date.available | 2016-02-15T12:13:07Z | |
dc.date.issued | 1984 | |
dc.identifier.issn | 1865-7125 | es |
dc.identifier.uri | http://hdl.handle.net/11441/34763 | |
dc.description.abstract | Nitrate reductase from spinach (Spinacia oleracea L.) leaves, which had been inactivated in vitro by incubation with N A D H and cyanide, was fully reactivated in minutes when irradiated in anaerobic conditions with red light in the presence o f m ethylene blue. Both the rate and the extent of reactivation increased with light intensity ( 6 to 100 W - m “ 2) and dye concentration (1 to 10 jiM). On the contrary, photoreactivation was com pletely abolished when N A D H or ethylenediaminetetra-acetic acid were present during irradiation. W e propose that methylene blue, when photo excited, exhibits a redox potential positive enough to reoxidise the CN~-reduced molybdenum complex settled in the inactive enzyme, thus causing its reactivation. On the other hand, prolonged irradiation o f nitrate reductase, under air and in the presence o f methylene blue, promoted an oxygen-dependent irreversible inactivation o f the two partial activities of the enzyme. This inactivation was markedly enhanced in 77% deuterated water and greatly prevented by azide, which indicates that singlet oxygen is the species primarily involved in the photooxidative inactivation o f the enzyme | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | De Gruyter | es |
dc.relation.ispartof | Zeitschrift für Naturforschung - Section C Journal of Biosciences,39C, 1079-1094 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Methylene Blue | es |
dc.subject | Nitrate Reductase | es |
dc.subject | Photosensitization | es |
dc.subject | Singlet Oxygen | es |
dc.subject | Spinacia | es |
dc.title | Red-light effects sensitized by methylene blue on nitrate reductase from spinach (Spinacia Oleracea L.) leaves | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología | es |
dc.relation.publisherversion | http://zfn.mpdl.mpg.de/data/Reihe_C/39/ZNC-1984-39c-1079.pdf | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/34763 |
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