dc.creator | Sánchez-Porro Álvarez, Cristina | |
dc.creator | Moreno Amador, María de Lourdes | |
dc.creator | Piubeli, Francine | |
dc.creator | Frias, Luciana | |
dc.creator | García Gutiérrez, María Teresa | |
dc.creator | Mellado Durán, María Encarnación | |
dc.date.accessioned | 2016-02-09T11:35:28Z | |
dc.date.available | 2016-02-09T11:35:28Z | |
dc.date.issued | 2011 | |
dc.identifier.issn | 1932-6203 | es |
dc.identifier.uri | http://hdl.handle.net/11441/34389 | |
dc.description.abstract | Background: Extensive use of phenolic compounds in industry has resulted in the generation of saline wastewaters that
produce significant environmental contamination; however, little information is available on the degradation of phenolic
compounds in saline conditions. Halomonas organivorans G-16.1 (CECT 5995T) is a moderately halophilic bacterium that we
isolated in a previous work from saline environments of South Spain by enrichment for growth in different pollutants,
including phenolic compounds. PCR amplification with degenerate primers revealed the presence of genes encoding ringcleaving
enzymes of the b-ketoadipate pathway for aromatic catabolism in H. organivorans.
Findings: The gene cluster catRBCA, involved in catechol degradation, was isolated from H. organivorans. The genes catA,
catB, catC and the divergently transcribed catR code for catechol 1,2-dioxygenase (1,2-CTD), cis,cis-muconate
cycloisomerase, muconolactone delta-isomerase and a LysR-type transcriptional regulator, respectively. The benzoate
catabolic genes (benA and benB) are located flanking the cat genes. The expression of cat and ben genes by phenol and
benzoic acid was shown by RT-PCR analysis. The induction of catA gene by phenol and benzoic acid was also probed by the
measurement of 1,2-CTD activity in H. organivorans growth in presence of these inducers. 16S rRNA and catA gene-based
phylogenies were established among different degrading bacteria showing no phylogenetic correlation between both
genes.
Conclusions/Significance: In this work, we isolated and determined the sequence of a gene cluster from a moderately
halophilic bacterium encoding ortho-pathway genes involved in the catabolic metabolism of phenol and analyzed the gene
organization, constituting the first report characterizing catabolic genes involved in the degradation of phenol in moderate
halophiles, providing an ideal model system to investigate the potential use of this group of extremophiles in the
decontamination of saline environments. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS One, 6 (6), 21049 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | bacterial protein | es |
dc.subject | benzoate catabolic A protein | es |
dc.subject | benzoate catabolic B protein | es |
dc.subject | benzoic acid | es |
dc.subject | catechol 1,2 dioxygenase | es |
dc.subject | isomerase | es |
dc.subject | phenol | es |
dc.subject | protein cat | es |
dc.title | Cloning, characterization and analysis of cat and ben genes from the phenol degrading halophilic bacterium Halomonas organivorans. | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0021049 | es |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0021049 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/34389 | |