dc.creator | Sánchez-Porro Álvarez, Cristina | |
dc.creator | León León, María José | |
dc.creator | Fernández, Ana Beatriz | |
dc.creator | Ghai, Rohit | |
dc.creator | Rodríguez Valera, Francisco | |
dc.creator | Ventosa Ucero, Antonio | |
dc.date.accessioned | 2016-02-08T12:09:32Z | |
dc.date.available | 2016-02-08T12:09:32Z | |
dc.date.issued | 2014 | |
dc.identifier.issn | 0099-2240 | es |
dc.identifier.uri | http://hdl.handle.net/11441/34309 | |
dc.description.abstract | Recent metagenomic studies on saltern ponds with intermediate salinities have determined that their microbial communities are dominated
by both Euryarchaeota and halophilic bacteria, with a gammaproteobacterium closely related to the genera Alkalilimnicola
and Arhodomonas being one of the most predominant microorganisms, making up to 15% of the total prokaryotic population.
Here we used several strategies and culture media in order to isolate this organism in pure culture. We report the isolation
and taxonomic characterization of this new, never before cultured microorganism, designated M19-40T, isolated from a saltern
located in Isla Cristina, Spain, using a medium with a mixture of 15% salts, yeast extract, and pyruvic acid as the carbon source.
Morphologically small curved cells (young cultures) with a tendency to form long spiral cells in older cultures were observed in
pure cultures. The organism is a Gram-negative, nonmotile bacterium that is strictly aerobic, non-endospore forming, heterotrophic,
and moderately halophilic, and it is able to grow at 10 to 25% (wt/vol) NaCl, with optimal growth occurring at 15% (wt/
vol) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that strain M19-40T has a low similarity
with other previously described bacteria and shows the closest phylogenetic similarity with species of the genera Alkalilimnicola
(94.9 to 94.5%), Alkalispirillum (94.3%), and Arhodomonas (93.9%) within the family Ectothiorhodospiraceae. The phenotypic,
genotypic, and chemotaxonomic features of this new bacterium showed that it constitutes a new genus and species, for which the
name Spiribacter salinus gen. nov., sp. nov., is proposed, with strain M19-40T ( CECT 8282T IBRC-M 10768T LMG
27464T) being the type strain. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Microbiology | es |
dc.relation.ispartof | Applied and Environmental Microbiology, 80 (13), 3850-3857 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Halophilic bacteria | es |
dc.subject | Isolation and characterization | es |
dc.subject | Microbial communities | es |
dc.subject | Optimal growth | es |
dc.subject | Phylogenetic analysis | es |
dc.subject | Taxonomic characterization | es |
dc.subject | Yeast extracts | es |
dc.title | From Metagenomics to Pure Culture: Isolation and Characterization of the Moderately Halophilic Bacterium Spiribacter salinus gen. nov., sp. nov. | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.publisherversion | http://dx.doi.org/10.1128/AEM.00430-14 | es |
dc.identifier.doi | 10.1128/AEM.00430-14 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/34309 | |