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Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair
dc.creator | Gaillard, Hélène | |
dc.creator | Tous Rivera, Cristina | |
dc.creator | Botet, Javier | |
dc.creator | González Aguilera, Cristina | |
dc.creator | Quintero, María José | |
dc.creator | Viladevall, Laia | |
dc.creator | García Rubio, María Luisa | |
dc.creator | Rodríguez Gil, Alfonso | |
dc.creator | Marín, Antonio | |
dc.creator | Ariño, Joaquín | |
dc.creator | Revuelta, José Luis | |
dc.creator | Chávez de Diego, Sebastián | |
dc.creator | Aguilera López, Andrés | |
dc.date.accessioned | 2015-10-02T11:17:18Z | |
dc.date.available | 2015-10-02T11:17:18Z | |
dc.date.issued | 2009 | |
dc.identifier.issn | 1553-7390 | es |
dc.identifier.issn | 1553-7404 | es |
dc.identifier.uri | http://hdl.handle.net/11441/29122 | |
dc.description.abstract | RNA polymerases frequently deal with a number of obstacles during transcription elongation that need to be removed for transcription resumption. One important type of hindrance consists of DNA lesions, which are removed by transcription-coupled repair (TC-NER), a specific sub-pathway of nucleotide excision repair. To improve our knowledge of transcription elongation and its coupling to TC-NER, we used the yeast library of non-essential knock-out mutations to screen for genes conferring resistance to the transcription-elongation inhibitor mycophenolic acid and the DNA-damaging agent 4-nitroquinoline-N-oxide. Our data provide evidence that subunits of the SAGA and Ccr4-Not complexes, Mediator, Bre1, Bur2, and Fun12 affect transcription elongation to different extents. Given the dependency of TC-NER on RNA Polymerase II transcription and the fact that the few proteins known to be involved in TC-NER are related to transcription, we performed an in-depth TC-NER analysis of a selection of mutants. We found that mutants of the PAF and Ccr4-Not complexes are impaired in TC-NER. This study provides evidence that PAF and Ccr4-Not are required for efficient TC-NER in yeast, unraveling a novel function for these transcription complexes and opening new perspectives for the understanding of TC-NER and its functional interconnection with transcription elongation. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Public Library of Science | es |
dc.relation.ispartof | PLoS Genetics, 5(2) | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | DNA transcription | es |
dc.subject | Gene expression | es |
dc.subject | DNA damage | es |
dc.subject | DNA repair | es |
dc.subject | Gene regulation | es |
dc.subject | Damage mechanics | es |
dc.subject | Transcription factors | es |
dc.subject | Yeast | es |
dc.title | Genome-wide analysis of factors affecting transcription elongation and DNA repair: a new role for PAF and Ccr4-not in transcription-coupled repair | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pgen.1000364 | es |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pgen.1000364 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/29122 |
Ficheros | Tamaño | Formato | Ver | Descripción |
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journal.pgen.1000364.pdf | 1.678Mb | [PDF] | Ver/ | |