Salimicrobium salexigens sp. nov., a moderately halophilic bacterium from salted hides

Abstract

Two Gram-positive, moderately halophilic bacteria, designated strains 29CMIT and 53CMI, were isolated from salted hides. Both strains were non-motile, strictly aerobic cocci, growing in the presence of 3-25 % (w/v) NaCl (optimal growth at 7.5-12.5 % [w/v] NaCl), between pH 5.0 and 10.0 (optimal growth at pH 7.5) and at temperatures between 15 and 40 ºC (optimal growth at 37 ºC). Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that both strains showed a similarity of 98.7 % and were closely related to species of the genus Salimicrobium, within the phylum Firmicutes. Strains 29CMIT and 53CMI exhibited 16S rRNA gene sequence similarity values of 97.9 % to 97.6 % with Salimicrobium album DSM 20748T, Salimicrobium halophilum DSM 4771T, Salimicrobium flavidum ISL-25T and Salimicrobium luteum BY-5T. The DNA G+C content was 50.7 mol% and 51.5 mol% for strains 29CMIT and 53CMI, respectively. The DNA-DNA hybridization between both strains was 98 %, whereas the values between strain 29CMIT and the species Salimicrobium album CCM 3517T, Salimicrobium luteum BY-5T, Salimicrobium flavidum ISL-25T and Salimicrobium halophilum CCM 4074T were 45 %, 28 %, 1