dc.creator | López Baena, Francisco Javier | |
dc.creator | Vinardell González, José María | |
dc.creator | Pérez Montaño, Francisco de Asís | |
dc.creator | Crespo Rivas, Juan Carlos | |
dc.creator | Bellogín Izquierdo, Ramón Andrés | |
dc.creator | Espuny Gómez, María del Rosario | |
dc.creator | Ollero Márquez, Francisco Javier | |
dc.date.accessioned | 2015-02-25T09:56:04Z | |
dc.date.available | 2015-02-25T09:56:04Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1465-2080 | es |
dc.identifier.issn | 1350-0872 | es |
dc.identifier.uri | http://hdl.handle.net/11441/22757 | |
dc.description.abstract | In this work we show that the Sinorhizobium fredii HH103 ttsI gene is essential for the expression
of the tts genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate
for the first time, to our knowledge, that the nod box preceding ttsI is necessary for Nops
secretion. TtsI is responsible for the transcriptional activation of nopX, nopA, rhcJ and rhcQ. We
confirm that the S. fredii HH103 ttsI gene is activated by NodD1 and repressed by NolR. In
contrast, NodD2 is not involved in the regulation of ttsI expression. Despite the dependence of
expression of both ttsI and nodA on NodD1 and flavonoids, clear differences in the capacity of
some flavonoids to activate these genes were found. The expression of the ttsI and nodA
genes was also sensitive to differences in the pH of the media. Secretion of Nops in the ttsI
mutant could not be complemented with a DNA fragment containing the ttsI gene and its nod
box, but it was restored when a plasmid harbouring the ttsI, rhcC2 and y4xK genes was
transferred to the mutant strain. The symbiotic effect of Nops secretion was host-dependent but
independent of the type of nodule formed by the host legume. Nops are beneficial in the symbiosis
with Glycine max and Glycyrrhiza uralensis, and detrimental in the case of the tropical legume
Erythrina variegata. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.relation.ispartof | Microbiology, 154: 1825–1836 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.publisherversion | 10.1099/mic.0.2007/016337-0 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/22757 | |