dc.creator | Rodríguez-González, Raquel | es |
dc.creator | Monsalve Guil, Loreto | es |
dc.creator | Jiménez Guerra, Álvaro | es |
dc.creator | Velasco-Ortega, Eugenio | es |
dc.creator | Moreno Muñoz, Jesús | es |
dc.creator | Núñez Márquez, Enrique | es |
dc.creator | Pérez, Román A. | es |
dc.creator | Gil, Javier | es |
dc.creator | Ortiz García, Iván | es |
dc.date.accessioned | 2024-07-02T15:32:13Z | |
dc.date.available | 2024-07-02T15:32:13Z | |
dc.date.issued | 2023-05 | |
dc.identifier.citation | Rodríguez-González, R., Monsalve Guil, L., Jiménez Guerra, Á., Velasco-Ortega, E., Moreno Muñoz, J., Núñez Márquez, E.,...,Ortiz García, I. (2023). Relevant aspects of titanium topography for osteoblastic adhesion and inhibition of bacterial colonization. Materials, 16 (9), 3553. https://doi.org/10.3390/ma16093553. | |
dc.identifier.issn | 1996-1944 | es |
dc.identifier.uri | https://hdl.handle.net/11441/161033 | |
dc.description.abstract | The influence of the surface topography of dental implants has been studied to optimize
titanium surfaces in order to improve osseointegration. Different techniques can be used to obtain
rough titanium, however, their effect on wettability, surface energy, as well as bacterial and cell adhe sion and differentiation has not been studied deeply. Two-hundred disks made of grade 4 titanium
were subjected to different treatments: machined titanium (MACH), acid-attacked titanium (AE),
titanium sprayed with abrasive alumina particles under pressure (GBLAST), and titanium that has
been treated with GBLAST and then subjected to AE (GBLAST + AE). The roughness of the different
treatments was determined by confocal microscopy, and the wettability was determined by the sessile
drop technique; then, the surface energy of each treatment was calculated. Osteoblast-like cells
(SaOs-2) were cultured, and alkaline phosphatase was determined using a colorimetric test. Likewise,
bacterial strains S. gordonii, S. oralis, A. viscosus, and E. faecalis were cultured, and proliferation on
the different surfaces was determined. It could be observed that the roughness of the GBLAST and
GBLAS + AE was higher, at 1.99 and 2.13 µm of Ra, with respect to the AE and MACH samples,
which were 0.35 and 0.20 µm, respectively. The abrasive treated surfaces showed lower hydrophilicity
but lower surface energy. Significant differences could be seen at 21 days between SaOS-2 osteoblastic
cell adhesion for the blasted ones and higher osteocalcin levels. However, no significant differences in
terms of bacterial proliferation were observed between the four surfaces studied, demonstrating the
insensitivity of bacteria to topography. These results may help in the search for the best topographies
for osteoblast behavior and for the inhibition of bacterial colonization. | es |
dc.format | application/pdf | es |
dc.format.extent | 13 p. | es |
dc.language.iso | eng | es |
dc.publisher | Mdpi | es |
dc.relation.ispartof | Materials, 16 (9), 3553. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Titanium | es |
dc.subject | Roughness | es |
dc.subject | Topography | es |
dc.subject | Osteoblasts | es |
dc.subject | Bacteria | es |
dc.subject | Periimplantitis | es |
dc.title | Relevant aspects of titanium topography for osteoblastic adhesion and inhibition of bacterial colonization | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Estomatología | es |
dc.relation.publisherversion | https://www.mdpi.com/1996-1944/16/9/3553 | es |
dc.identifier.doi | 10.3390/ma16093553 | es |
dc.contributor.group | Universidad de Sevilla. CTS618: Investigación Básica y Clínica en Implantología Oral | es |
dc.journaltitle | Materials | es |
dc.publication.volumen | 16 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 3553 | es |