dc.creator | Baus Domínguez, María | es |
dc.creator | Gómez-Díaz, Raquel | es |
dc.creator | Martín-Lozano, Guiomar | es |
dc.creator | Iglesias-Martín, Fernando | es |
dc.creator | Gutiérrez Pérez, José Luis | es |
dc.creator | Torres-Lagares, Daniel | es |
dc.creator | Serrera Figallo, María de los Ángeles | es |
dc.creator | Gutiérrez Corrales, Aída | es |
dc.date.accessioned | 2024-06-19T12:35:14Z | |
dc.date.available | 2024-06-19T12:35:14Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Baus Domínguez, M., Gómez-Díaz, R., Martín-Lozano, G., Iglesias-Martín, F., Gutiérrez Pérez, J.L., Torres-Lagares, D.,...,Gutiérrez Corrales, A. (2023). Detection of NOTCH1 mutations in paraffin samples in patients with potentially malignant lesions. Journal of Clinical and Experimental Dentistry, 15 (3), e177. https://doi.org/10.4317/jced.60293. | |
dc.identifier.issn | 1989-5488 | es |
dc.identifier.uri | https://hdl.handle.net/11441/160690 | |
dc.description.abstract | Background: On certain occasions, oral cancer is preceded by potentially malignant lesions. The degree of dysplasia in Guinea pigs attempts to determine the risk of developing a malignant lesion. The search for genetic mutations,
biomarkers, as a more truthful and reproducible diagnostic tool, tries to fill the gaps in the anatomopathological
study. In this line, the present retrospective case-control study is based on the detection of known mutations of
the NOTCH1 gene in biopsied samples of potentially malignant lesions from 22 patients who attend the Oral and
Maxillofacial Surgery service of the Virgen del Rocío University Hospital.
Material and Methods: DNA extraction after dewaxing of the samples using the Minikit QIAamp DNA FFPE tissue
extraction kit with extraction kit (reference 56404) of QIAGEN. Subsequently, with the DNA obtained, 4 amplification reactions were carried out using enzyme polymerase. Before sequencing the samples, they were purified
with the ExoSAP-IT for PCR product cleaning kit of the INVITROGEN brand. Finally, to detect somatic mutations
in NOTCH1, TaqMan Mutation Detection Assays was used and for the analysis of mutations we worked with the
Mutation Detector software.
Results: the mutation for NOTCH1 is not detected, the studied sample does not present the mutation, or it is below
the limits of detection of the software.
Conclusions: In the clinical setting of the sample, the NOTCH1 mutation seems to be not very frequent, although
NOTCH1 has been described as a gene related to oral cancer in other geographical settings. | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.relation.ispartof | Journal of Clinical and Experimental Dentistry, 15 (3), e177. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Oral cancer | es |
dc.subject | NOTCH1 | es |
dc.subject | Mutations | es |
dc.title | Detection of NOTCH1 mutations in paraffin samples in patients with potentially malignant lesions | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Estomatología | es |
dc.relation.projectID | PI15-01834 | es |
dc.identifier.doi | 10.4317/jced.60293 | es |
dc.journaltitle | Journal of Clinical and Experimental Dentistry | es |
dc.publication.volumen | 15 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | e177 | es |
dc.contributor.funder | Instituto de Salud Carlos III | es |