Fabrication, physical-chemical and biological characterization of retinol-loaded poly(vinyl alcohol) electrospun fiber mats for wound healing applications

Abstract

Nowadays, there exists a huge interest in producing innovative, high-performance, biofunctional, and cost-efficient electrospun biomaterials based on the association of biocompatible polymers with bioactive molecules. Such materials are well-known to be promising candidates for three-dimensional biomimetic systems for wound healing applications because they can mimic the native skin microenvironment; however, many open questions such as the interaction mechanism between the skin and the wound dressing material remain unclear. Recently, several biomolecules were intended for use in combination with poly(vinyl alcohol) (PVA) fiber mats to improve their biological response; nevertheless, retinol, an important biomolecule, has not been combined yet with PVA to produce tailored and biofunctional fiber mats. Based on the abovementioned concept, the present work reported the fabrication of retinol-loaded PVA electrospun fiber mats (RPFM) with a variable content of retinol (0 ≤ Ret ≤ 25 wt.%), and their physical–chemical and biological characterization. SEM results showed that fiber mats exhibited diameters distribution ranging from 150 to 225 nm and their mechanical properties were affected with the increasing of retinol concentrations. In addition, fiber mats were able to release up to 87% of the retinol depending on both the time and the initial content of retinol. The cell culture results using primary mesenchymal stem cell cultures proved the biocompatibility of RPFM as confirmed by their effects on cytotoxicity (low level) and proliferation (high rate) in a dose-dependent manner. Moreover, the wound healing assay suggested that the optimal RPFM with retinol content of 6.25 wt.% (RPFM-1) enhanced the cell migratory activity without altering its morphology. Accordingly, it is demonstrated that the fabricated RPFM with retinol content below the threshold 0 ≤ Ret ≤ 6.25 wt.% would be an appropriate system for skin regenerative application.