dc.creator | Pérez, David | es |
dc.creator | Moyá Morán, María Luisa | es |
dc.creator | Bautista, María | es |
dc.creator | León, Rosa | es |
dc.creator | Molina-Márquez, Ana | es |
dc.creator | Vila, Marta | es |
dc.creator | Romero Azogil, Lucía | es |
dc.creator | Benito Hernández, Elena María | es |
dc.creator | García Martín, María de Gracia | es |
dc.creator | Valle Rosado, Iván | es |
dc.creator | Romero Balestra, Fernando | es |
dc.creator | Huertas Sánchez, Pablo | es |
dc.creator | López-Cornejo, María del Pilar | es |
dc.date.accessioned | 2024-05-27T14:11:23Z | |
dc.date.available | 2024-05-27T14:11:23Z | |
dc.date.issued | 2023-02-23 | |
dc.identifier.citation | Pérez, D., Moyá Morán, M.L., Bautista, M., León, R., Molina-Márquez, A., Vila, M.,...,López-Cornejo, M.d.P. (2023). A novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cells. Colloids and surfaces B-biointerfaces, 224, 113219. https://doi.org/10.1016/j.colsurfb.2023.113219. | |
dc.identifier.issn | 1873-4367 | es |
dc.identifier.issn | 0927-7765 | es |
dc.identifier.uri | https://hdl.handle.net/11441/159043 | |
dc.description.abstract | The design and preparation of new vectors to transport genetic material and increase the transfection efficiency
continue being an important research line. Here, a novel biocompatible sugar-based polymer derived from Dmannitol has been synthesized to be used as a gene material nanocarrier in human (gene transfection) and
microalga cells (transformation process). Its low toxicity allows its use in processes with both medical and industrial applications. A multidisciplinary study about the formation of polymer/p-DNA polyplexes has been
carried out using techniques such as gel electrophoresis, zeta potential, dynamic light scattering, atomic force
microscopy, and circular dichroism spectroscopy. The nucleic acids used were the eukaryotic expression plasmid
pEGFP-C1 and the microalgal expression plasmid Phyco69, which showed different behaviors. The importance of
DNA supercoiling in both transfection and transformation processes was demonstrated. Better results were obtained in microalga cells nuclear transformation than in human cells gene transfection. This was related to the
plasmid’s conformational changes, in particular to their superhelical structure. It is noteworthy that the same
nanocarrier has been used with eukaryotic cells from both human and microalga. | es |
dc.description.sponsorship | Consejería de Conocimiento, Innovación y Universidades de la Junta de Andalucía FQM-206, FQM- 274, FQM-135 y P20–01234 | es |
dc.description.sponsorship | VI Plan Propio Universidad de Sevilla PP2019/00000748 | es |
dc.format | application/pdf | es |
dc.format.extent | 9 | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier Science BV | es |
dc.relation.ispartof | Colloids and surfaces B-biointerfaces, 224, 113219. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | pDNA | es |
dc.subject | Gene therapy | es |
dc.subject | Transformation process in algae | es |
dc.subject | Supercoiling structure | es |
dc.subject | Biocompatible polymer | es |
dc.subject | Microalgae | es |
dc.title | A novel biocompatible polymer derived from D-mannitol used as a vector in the field of genetic engineering of eukaryotic cells | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Física | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.colsurfb.2023.113219 | es |
dc.identifier.doi | 10.1016/j.colsurfb.2023.113219 | es |
dc.journaltitle | Colloids and surfaces B-biointerfaces | es |
dc.publication.volumen | 224 | es |
dc.publication.initialPage | 113219 | es |
dc.contributor.funder | Consejería de Conocimiento, Innovación y Universidades de la Junta de Andalucía | es |
dc.contributor.funder | Universidad de Sevilla | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |