Soil microbial community composition and functions are disrupted by fire and land use in a Mediterranean woodland

Abstract

The intersection of fire, land use transformations, and climate change is putting Mediterranean climate-type ecosystems at risk of soil degradation and loss of ecosystem services. Ondik et al. (2022b) showed that in a Mediterranean dry sclerophyll woodland of South Australia, high severity fire and clearing and grazing practices impacted both physicochemical and biological soil quality indicators. Building upon the work of Ondik et al. (2022b) this study aims to 1) identify soil physicochemical properties impacted by fire and land management that are indirect drivers of changes to soil microbial community composition and 2) determine whether the observed changes to soil microbial community composition affect soil microbial functions. Via a redundancy analysis, we identified fire and management-induced changes to pH, soil water repellency, nutrient stoichiometry, and total nutrient content as significant drivers of the composition of soil microbial communities. We then measured basal respiration, substrate induced respiration, and the carbon mineralisation quotient, and calculated functional trait distributions among microbial communities by linking 16S and 18S rRNA sequences to respiration modes and functional guilds, respectively. We found that fire reduced soil microbial respiration and the relative abundance (RA) of microbial symbionts, anaerobic bacteria, and microaerophilic bacteria, while increasing the RA of aerobic bacteria. Furthermore, management increased the RA of post-fire ectomycorrhizal fungi and may have reduced pathogenic load, microbial efficiency, and wood saprotrophs, while increasing litter, soil, and other saprotrophic species that are adapted to grasslands. This study shows that, through changes to microbial community composition, high severity wildfire and land management affected soil respiration rates, bacterial modes of respiration, prevalence of symbiotic bacteria and fungi, and microbial substrate preference. Having identified the main physicochemical drivers of changes to microbial community composition, we provide valuable insights into how fire and land management can impact soils in Mediterranean woodland.