Artículo
Rio Tinto as a niche for acidophilus enzymes of industrial relevance
Autor/es | Daddaoua, Abdelali
Álvarez Núñez, Consolación ![]() ![]() ![]() ![]() ![]() ![]() ![]() Oggerin, Monika Rodríguez, Nuria Duque, Estrella Amils, Ricardo Armengaud, Jean Segura, Ana Ramos, Juan Luis |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2023-05 |
Fecha de depósito | 2024-02-26 |
Publicado en |
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Resumen | Lignocellulosic residues are amongst the most abundant waste products on Earth. Therefore, there is an increasing interest in the utilization of these residues for bioethanol production and for biorefineries to produce ... Lignocellulosic residues are amongst the most abundant waste products on Earth. Therefore, there is an increasing interest in the utilization of these residues for bioethanol production and for biorefineries to produce compounds of industrial interest. Enzymes that breakdown cellulose and hemicellulose into oligomers and monosaccharides are required in these processes and cellulolytic enzymes with optimum activity at a low pH area are desirable for industrial processes. Here, we explore the fungal biodiversity of Rıo Tinto, the largest acidic ecosystem on Earth, as far as the secretion of cellulolytic enzymes is concerned. Using colorimetric and industrial substrates, we show that a high proportion of the fungi present in this extremophilic environment secrete a wide range of enzymes that are able to hydrolyze cellulose and hemicellulose at acidic pH (4.5–5). Shotgun proteomic analysis of the secretomes of some of these fungi has identified different cellulases and hemicellulolytic enzymes as well as a number of auxiliary enzymes. Supplementation of pre-industrial cocktails from Myceliophtora with Rio Tinto secretomes increased the amount of monosaccharides released from corn stover or sugar cane straw. We conclude that the Rio Tinto fungi display a good variety of hydrolytic enzymes with high industrial potential. |
Agencias financiadoras | Consejo Superior de Investigaciones Científicas (CSIC) Ministerio de Ciencia e Innovación (MICIN). España |
Identificador del proyecto | AEP084
![]() RTI2018-094370-B-I00 ![]() |
Cita | Daddaoua, A., Álvarez Núñez, C., Oggerin, M., Rodríguez, N., Duque, E., Amils, R.,...,Ramos, J.L. (2023). Rio Tinto as a niche for acidophilus enzymes of industrial relevance. Microbial Biotechnology, 16 (5), 1069-1086. https://doi.org/https://doi.org/10.1111/1751-7915.14192. |
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Rio Tinto.pdf | 707.1Kb | ![]() | Ver/ | Versión publicada |