Artículo
A proxitome-RNA-capture approach reveals that processing bodies repress co-regulated hub genes
Autor/es | Liu, Chen
Mentzelopoulou, Andriani Hatzianestis, Ioannis H. Tzagkarakis, Epameinondas Skaltsogiannis, Vasileios Ma, Xuemin Michalopoulou, Vassiliki A. Romero Campero, Francisco José Romero Losada, Ana Belén Sarris, Panagiotis F. Marhavy, Peter Bolter, Bettina Kanterakis, Alexandros Gutiérrez Beltrán, Emilio Moschou, Panagiotis N. |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular Universidad de Sevilla. Departamento de Ciencias de la Computación e Inteligencia Artificial |
Fecha de publicación | 2023-11-16 |
Fecha de depósito | 2024-02-21 |
Publicado en |
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Resumen | Cellular condensates are usually ribonucleoprotein assemblies with liquid- or solid-like properties. Because these subcellular structures lack a delineating membrane, determining their compositions is difficult. Here we ... Cellular condensates are usually ribonucleoprotein assemblies with liquid- or solid-like properties. Because these subcellular structures lack a delineating membrane, determining their compositions is difficult. Here we describe a proximity-biotinylation approach for capturing the RNAs of the condensates known as processing bodies (PBs) in Arabidopsis (Arabidopsis thaliana). By combining this approach with RNA detection, in silico, and high-resolution imaging approaches, we studied PBs under normal conditions and heat stress. PBs showed a much more dynamic RNA composition than the total transcriptome. RNAs involved in cell wall development and regeneration, plant hormonal signaling, secondary metabolism/defense, and RNA metabolism were enriched in PBs. RNA-binding proteins and the liquidity of PBs modulated RNA recruitment, while RNAs were frequently recruited together with their encoded proteins. In PBs, RNAs follow distinct fates: in small liquid-like PBs, RNAs get degraded while in more solid-like larger ones, they are stored. PB properties can be regulated by the actin-polymerizing SCAR (suppressor of the cyclic AMP)-WAVE (WASP family verprolin homologous) complex. SCAR/WAVE modulates the shuttling of RNAs between PBs and the translational machinery, thereby adjusting ethylene signaling. In summary, we provide an approach to identify RNAs in condensates that allowed us to reveal a mechanism for regulating RNA fate. |
Agencias financiadoras | Carl Tryggers Stiftelse foer Vetenskaplig Forskning Svenska Forskningsradet Formas European Union (UE) Helge Ax:son Johnsons stiftelse Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | 15:336
17:336 2017-00938 872969 F19-0419 BIO2017-84066-R PID2020-119737GA-I00 ProyExcel_00587 |
Cita | Liu, C., Mentzelopoulou, A., Hatzianestis, I.H., Tzagkarakis, E., Skaltsogiannis, V., Ma, X.,...,Moschou, P.N. (2023). A proxitome-RNA-capture approach reveals that processing bodies repress co-regulated hub genes. Plant Cell, 36 (3), 559-584. https://doi.org/10.1093/plcell/koad288. |
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A proxitome-RNA.pdf | 2.834Mb | [PDF] | Ver/ | Versión publicada |