Inoculating native microorganisms improved soil function and altered the microbial composition of a degraded soil

Abstract

Restoration managers inoculate microorganisms to enhance soil function and improve restoration success, but the efficacy of these inoculations in real-world conditions is still unclear. We conducted a field experiment to test whether applying extruded seed pellets inoculated with native microbes affected soil properties related to ecosystem function in severely degraded mine soil. We found that inoculating with bacteria did not affect soil carbon, metabolic quotient (a measure of microbial stress), or basal respiration, but increased soil nitrogen by 75%, substrate-induced respiration by 147% and reduced carbon-to-nitrogen ratio by 44% compared to the control. This suggests that the bacteria inoculant contained free-living N fixers that increased the soil N content. Thus, inoculating with bacteria could supplement nitrogen fertilizers in degraded soils during soil restoration. However, we found that inoculating with a mix of bacteria and cyanobacteria did not affect any of the soil properties. This finding is counter to results in laboratory studies, suggesting that field tests are critical for understanding real-world outcomes of microbial inoculation. Finally, we found that soil microbial composition was changed by the inoculation with a mix of bacteria and cyanobacteria. None of the treatments significantly changed the diversity of soil microbial communities. Our data suggest that microbial inoculation could improve some aspects of ecosystem function and thus provide beneficial effects that might facilitate restoration of degraded sites.