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Artículo
Root phosphoenolpyruvate carboxylase activity is essential for Sorghum bicolor tolerance to ammonium nutrition
Autor/es | Marín Peña, A. J.
Vega Mas, Izargi Busturia, I. Osa Fernández, Clara de la González Moro, María Begoña Monreal Hermoso, José Antonio Marino, Daniel |
Departamento | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología |
Fecha de publicación | 2024-01 |
Fecha de depósito | 2024-02-16 |
Publicado en |
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Resumen | Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is an enzyme family with pivotal roles in plant carbon and
nitrogen metabolism. A main role for non-photosynthetic PEPC is as anaplerotic enzyme to load tricarboxylic
acid ... Phosphoenolpyruvate carboxylase (PEPC; EC 4.1.1.31) is an enzyme family with pivotal roles in plant carbon and nitrogen metabolism. A main role for non-photosynthetic PEPC is as anaplerotic enzyme to load tricarboxylic acid (TCA) cycle with carbon skeletons that compensate the intermediates diverted for biomolecule synthesis such as amino acids. When plants are grown under ammonium (NH4 +) nutrition, the excessive uptake of NH4 + often provokes a stress situation. When plants face NH4 + stress, N assimilation is greatly induced and thus, requires the supply of carbon skeletons coming from TCA cycle. In this work, we addressed the importance of root PEPC and TCA cycle for sorghum (Sorghum bicolor L. Moench), a C4 cereal crop, grown under ammonium nutrition. To do so, we used RNAi sorghum lines that display a decrease expression of SbPPC3 (Ppc3 lines), the main root PEPC isoform, and reduced root PEPC activity. SbPPC3 silencing provoked ammonium hypersensitivity, meaning lower biomass accumulation in Ppc3 respect to WT plants when growing under ammonium nutrition. The silenced plants presented a deregulation of primary metabolism as highlighted by the accumulation of NH4 + in the root and the alteration of normal TCA functioning, which was evidenced by the accumulation of organic acids in the root under ammonium nutrition. Altogether, our work evidences the importance of non-photosynthetic PEPC, and root TCA cycle, in sorghum to deal with high external NH4 + availability. |
Agencias financiadoras | Gobierno Vasco Junta de Andalucía European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Ministerio de Ciencia e Innovación (MICIN). España Agencia Estatal de Investigación. España |
Identificador del proyecto | IT1560-22
FEDER 2014–2020 - US- 1251626 AGL2016-75413-P BIO2017-84035-R PRE2018-085268 |
Cita | Marín Peña, A.J., Vega Mas, I., Busturia, I., Osa Fernández, C.d.l., González Moro, M.B., Monreal Hermoso, J.A. y Marino, D. (2024). Root phosphoenolpyruvate carboxylase activity is essential for Sorghum bicolor tolerance to ammonium nutrition. Plant Physiology and Biochemistry, 206, 108312. https://doi.org/10.1016/j.plaphy.2023.108312. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Journal of Ecology - 2023 - Homet ... | 1.624Mb | [PDF] | Ver/ | |
1-s2.0-S0981942823008239-mmc1.pdf | 113.0Kb | [PDF] | Ver/ | Apéndice A. Datos complementarios |