Artículo
Biodegradable particles for protein delivery: estimation of the release kinetics inside cells
Autor/es | Zyuzin, Mikhail V.
Hartmann, Raimo Timin, Alexander S. Carregal Romero, Susana Parak, Wolfgang J. Escudero Belmonte, Alberto |
Departamento | Universidad de Sevilla. Departamento de Química Inorgánica |
Fecha de publicación | 2022-08 |
Fecha de depósito | 2024-02-14 |
Publicado en |
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Resumen | A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules
with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine)
and ... A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine) and SiO2 was developed. The capsules were loaded with model proteins such as ovalbumin and green fluorescent protein (GFP), and the protein release profile inside cells (either macrophages or HeLa cells) after endocytosis was analysed. Both, protein loading and release kinetics were evaluated by analysing confocal laser scanning microscopy images using MatLab and CellProfiler software. Our results indicate that silica capsules showed the most efficient release of proteins as cargo molecules within 48 h, as compared to their polymeric counterparts. This developed method for the analysis of the intracellular cargo release kinetics from carrier structures could be used in the future for a better control of drug release profiles. |
Agencias financiadoras | Ministerio de Ciencia, Innovación y Universidades (MICINN). España Universidad de Sevilla Deutsche Forschungsgemeinschaft / German Research Foundation (DFG) Russian Federation |
Identificador del proyecto | MDM-2017 -0720
VI PPIT-US DFG PA 794/21-2 |
Cita | Zyuzin, M.V., Hartmann, R., Timin, A.S., Carregal Romero, S., Parak, W.J. y Escudero Belmonte, A. (2022). Biodegradable particles for protein delivery: estimation of the release kinetics inside cells. Biomaterials Advances, 139, 212966. https://doi.org/10.1016/j.bioadv.2022.212966. |
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