Artículo
Sinorhizobium fredii HH103 nolR and nodD2 mutants gain capacity for infection thread invasion of Lotus japonicus Gifu and Lotus burttii
Autor/es | Acosta Jurado, Sebastián
Rodríguez-Navarro, Dulce Nombre Kawaharada, Yasuyuki Rodríguez Carvajal, Miguel Ángel Gil Serrano, Antonio Miguel Soria Díaz, María Eugenia Pérez Montaño, Francisco de Asís Fernández Perea, Juan Niu, Yanbo Alias Villegas, Cynthia |
Departamento | Universidad de Sevilla. Departamento de Microbiología |
Fecha de publicación | 2019-03-06 |
Fecha de depósito | 2024-02-12 |
Publicado en |
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Resumen | Sinorhizobium fredii HH103 RifR, a broad-host-range rhizobial strain, forms ineffective nodules with Lotus japonicus but induces nitrogen-fixing nodules in Lotus burttii roots that are infected by intercellular entry. Here ... Sinorhizobium fredii HH103 RifR, a broad-host-range rhizobial strain, forms ineffective nodules with Lotus japonicus but induces nitrogen-fixing nodules in Lotus burttii roots that are infected by intercellular entry. Here we show that HH103 RifR nolR or nodD2 mutants gain the ability to induce infection thread formation and to form nitrogen-fixing nodules in L. japonicus Gifu. Microscopy studies showed that the mode of infection of L. burttii roots by the nodD2 and nolR mutants switched from intercellular entry to infection threads (ITs). In the presence of the isoflavone genistein, both mutants overproduced Nod-factors. Transcriptomic analyses showed that, in the presence of Lotus japonicus Gifu root exudates, genes related to Nod factors production were overexpressed in both mutants in comparison to HH103 RifR. Complementation of the nodD2 and nolR mutants provoked a decrease in Nod-factor production, the incapacity to form nitrogen-fixing nodules with L. japonicus Gifu and restored the intercellular way of infection in L. burttii. Thus, the capacity of S. fredii HH103 RifR nodD2 and nolR mutants to infect L. burttii and L. japonicus Gifu by ITs and fix nitrogen L. japonicus Gifu might be correlated with Nod-factor overproduction, although other bacterial symbiotic signals could also be involved |
Agencias financiadoras | Junta de Andalucía. P07-CVI-07500 |
Cita | Acosta Jurado, S., Rodríguez-Navarro, D.N., Kawaharada, Y., Rodríguez Carvajal, M.Á., Gil Serrano, A.M., Soria Díaz, M.E.,...,Alias Villegas, C. (2019). Sinorhizobium fredii HH103 nolR and nodD2 mutants gain capacity for infection thread invasion of Lotus japonicus Gifu and Lotus burttii. Environmental microbiology, 21 (5), 1718-1739. https://doi.org/10.1111/1462-2920.14584. |
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