Artículo
Au@16-pH-16/miR-21 mimic nanosystem: An efficient treatment for obesity through browning and thermogenesis induction
Autor/es | Lhamyani, Said
Gentile, Adriana Mariel Mengual Mesa, María Grueso Molina, Elia María Giráldez Pérez, Rosa María Fernandez Garcia, José Carlos Vega Rioja, Antonio Clemente Postigo, Mercedes El Bekay, Rajaa |
Departamento | Universidad de Sevilla. Departamento de Química Física Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2024-02 |
Fecha de depósito | 2024-01-11 |
Publicado en |
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Resumen | Despite the abundance of registered clinical trials worldwide, the availability of effective drugs for obesity treatment is limited due to their associated side effects. Thus, there is growing interest in therapies that ... Despite the abundance of registered clinical trials worldwide, the availability of effective drugs for obesity treatment is limited due to their associated side effects. Thus, there is growing interest in therapies that stimulate energy expenditure in white adipose tissue. Recently, we demonstrated that the delivery of a miR-21 mimic using JetPEI effectively inhibits weight gain in an obese mouse model by promoting metabolism, browning, and thermogenesis, suggesting the potential of miR-21 mimic as a treatment for obesity. Despite these promising results, the implementation of more advanced delivery system techniques for miR-21 mimic would greatly enhance the advancement of safe and efficient treatment approaches for individuals with obesity in the future. Our objective is to explore whether a new delivery system based on gold nanoparticles and Gemini surfactants (Au@16-ph-16) can replicate the favorable effects of the miR-21 mimic on weight gain, browning, and thermogenesis. We found that dosages as low as 0.2 μg miR-21 mimic /animal significantly inhibited weight gain and induced browning and thermogenic parameters. This was evidenced by the upregulation of specific genes and proteins associated with these processes, as well as the biogenesis of beige adipocytes and mitochondria. Significant increases in miR-21 levels were observed in adipose tissue but not in other tissue types. Our data indicates that Au@16-ph-16 could serve as an effective delivery system for miRNA mimics, suggesting its potential suitability for the development of future clinical treatments against obesity. |
Agencias financiadoras | Instituto de Salud Carlos III (ISCIII) European Union (UE) Junta de Andalucía European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Universidad de Sevilla Universidad de Málaga Ministerio de Economía y Competitividad (MINECO). España |
Identificador del proyecto | PI21/01924
PI18/00785 PI20-01274 PI-0235-2021 DOC-01138 RC-0006-2020 C-0060-2018 2021/00001297 PI-0092–2017 RC-0001-2021 SAF2014-60649-JIN |
Cita | Lhamyani, S., Gentile, A.M., Mengual Mesa, M., Grueso Molina, E.M., Giráldez Pérez, R.M., Fernandez Garcia, J.C.,...,El Bekay, R. (2024). Au@16-pH-16/miR-21 mimic nanosystem: An efficient treatment for obesity through browning and thermogenesis induction. Biomedicine & Pharmacotherapy, 171, 116104. https://doi.org/10.1016/j.biopha.2023.116104. |
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