dc.creator | Borroni, Davide | es |
dc.creator | Bonzano, Chiara | es |
dc.creator | Sánchez González, José María | es |
dc.creator | Rachwani-Anil, Rahul | es |
dc.creator | Zamorano Martín, Francisco | es |
dc.creator | Pereza Nieves, Jorge | es |
dc.creator | Traverso, Carlo Enrico | es |
dc.creator | García Lorente, María | es |
dc.creator | Rodríguez-Calvo-de-Mora, Marina | es |
dc.creator | Espósito, Alfonso | es |
dc.creator | Godin, Fernando | es |
dc.creator | Rocha de Lossada, Carlos | es |
dc.date.accessioned | 2023-12-14T17:42:59Z | |
dc.date.available | 2023-12-14T17:42:59Z | |
dc.date.issued | 2023-07 | |
dc.identifier.citation | Borroni, D., Bonzano, C., Sánchez González, J.M., Rachwani-Anil, R., Zamorano Martín, F., Pereza Nieves, J.,...,Rocha de Lossada, C. (2023). Shotgun metagenomic sequencing in culture negative microbial keratitis. European Journal of Ophthalmology, 33 (4), 1589-1595. https://doi.org/10.1177/11206721221149077. | |
dc.identifier.issn | 1120-6721 | es |
dc.identifier.issn | 1724-6016 | es |
dc.identifier.uri | https://hdl.handle.net/11441/152524 | |
dc.description.abstract | Purpose: To evaluate the microbiota of culture negative Corneal Impression Membrane (CIM) microbial keratitis samples with the use of shotgun metagenomics analysis. Methods: DNA of microbial keratitis samples were collected with CIM and extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre). DNA was fragmented by sonication into fragments of 300 to 400 base pairs (bp) using Bioruptor® (Diagenode, Belgium) and then used as a template for library preparation. DNA libraries were sequenced on Illumina® HiSeq2500. The resulting reads were quality controlled, trimmed and mapped against the human reference genome. The unmapped reads were taxonomically classified using the Kraken software. Results: 18 microbial keratitis samples were included in the study. Brevundimonas diminuta was found in 5 samples while 6 samples showed the presence of viral infections. Cutibacterium acnes, Staphylococcus aureus, Moraxella lacunata and Pseudomonas alcaligenes were also identified as the presumed putative cause of the infection in 7 samples. Conclusions: Shotgun sequencing can be used as a diagnostic tool in microbial keratitis samples. This diagnostic method expands the available tests to diagnose eye infections and could be clinically significant in culture negative samples. | es |
dc.format | application/pdf | es |
dc.format.extent | 17 p. | es |
dc.language.iso | eng | es |
dc.publisher | Wichtig Publishing Srl | es |
dc.relation.ispartof | European Journal of Ophthalmology, 33 (4), 1589-1595. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Brevundimonas diminuta | es |
dc.subject | Culture negative | es |
dc.subject | Keratitis | es |
dc.subject | Metagenomics | es |
dc.title | Shotgun metagenomic sequencing in culture negative microbial keratitis | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Física de la Materia Condensada | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Cirugía | es |
dc.relation.publisherversion | https://doi.org/10.1177/11206721221149077 | es |
dc.identifier.doi | 10.1177/11206721221149077 | es |
dc.journaltitle | European Journal of Ophthalmology | es |
dc.publication.volumen | 33 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 1589 | es |
dc.publication.endPage | 1595 | es |