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dc.creatorBaus Domínguez, Maríaes
dc.creatorGómez-Díaz, R.es
dc.creatorGutiérrez Pérez, José Luises
dc.creatorTorres-Lagares, Danieles
dc.creatorMachuca-Portillo, Guillermoes
dc.creatorSerrera Figallo, María de los Ángeleses
dc.date.accessioned2023-11-23T14:46:38Z
dc.date.available2023-11-23T14:46:38Z
dc.date.issued2022-06-08
dc.identifier.citationBaus Domínguez, M., Gómez-Díaz, R., Gutiérrez Pérez, J.L., Torres-Lagares, D., Machuca-Portillo, G. y Serrera Figallo, M.d.l.Á. (2022). Metallothioneins in dental implant treatment failure and periodontitis in patients with Down's syndrome: validation of results. GENES, 13 (6). https://doi.org/10.3390/genes13061028.
dc.identifier.issn2073-4425es
dc.identifier.urihttps://hdl.handle.net/11441/151449
dc.description.abstractMetallothioneins (MTs) are low molecular weight cysteine-rich proteins that can bind up to seven zinc ions. Among their numerous functions, MTs appear to act as protectors against oxidative and inflammatory injury. In our first published study, we reported downregulation of the isoforms MT1B (fold distance (FD) −2. 95; p = 0.0024), MT1F (FD −1.72; p = 0.0276), MT1X (FD −3.09; p = 0.0021), MT1H (FD −2.39; p = 0.0018), MT1M (FD −2.37; p = 0.0092), MT1L (FD −2. 55; p = 0.0048), MT1E (FD −2.71; p = 0.0014), MT2A (FD −2.35; p = 0.0072), MT1G (FD −2.24; p = 0.0118), and MT1A (FD −2.82; p = 0.0023) by comparing Down’s syndrome patients with periodontal disease and implant failure to those without periodontal disease and with a positive progression of their implants. In this gene validation study, we intended to verify the results of our first gene expression analysis. Materials and Methods: In our retrospective case–control study, we performed retrotranscription (RT-qPCR) of 11 RNA-to-cDNA samples using the SuperScript™ VILO™ kit (50; reference 1,176,605) from Thermo Fisher. We conducted the study using the real-time PCR technique on the q-PCR ViiA 7 platform from Thermo Fisher. We chose the format of the Taqman Array Plate 16 Plus (reference 4,413,261) from Thermo Fisher, which accommodates 12 genes plus four controls (GAPDH, 18S, ACTB, and HPRT1). We conducted the analysis of the plates using the Thermo Fisher Cloud Web Software. Results: The results obtained through gene validation analysis show that in PD+RI+ patients, the genes encoding the isoforms MT1F (FD 0.3; p = 0.039), MT1X (FD 338; p = 0.0078), MT1E (FD 307; p = 0.0358), and MT2A (FD 252; p = 0.0428) continue to show downregulation, whereas MT1B (FD 2.75; p = 0.580), MT1H (FD 281; p = 0.152), MT1L (FD 354; p = 0.0965), and MT1G (FD 336; p = 0.0749) no longer show statistically significant results.es
dc.formatapplication/pdfes
dc.format.extent11 p.es
dc.language.isoenges
dc.publisherMDPIes
dc.relation.ispartofGENES, 13 (6).
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectDown’s syndromees
dc.subjectPeriodontal diseasees
dc.subjectBone biologyes
dc.subjectClinical outcomeses
dc.subjectGene expressiones
dc.subjectValidationes
dc.subjectSystemic diseasees
dc.titleMetallothioneins in dental implant treatment failure and periodontitis in patients with Down's syndrome: validation of resultses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Estomatologíaes
dc.relation.projectIDPI-0081-2016es
dc.relation.publisherversionhttps://www.mdpi.com/2073-4425/13/6/1028es
dc.identifier.doi10.3390/genes13061028es
dc.journaltitleGENESes
dc.publication.volumen13es
dc.publication.issue6es
dc.contributor.funderConsejería de Salud de la Junta de Andalucía (España)es

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